Standard Name	RTT101 1
Systematic Name	YJL047C
Alias	CUL8 2
Feature Type	ORF, Verified
Description	Cullin subunit of a Roc1p-dependent E3 ubiquitin ligase complex with a role in anaphase progression; implicated in Mms22-dependent DNA repair; involved with Mms1p in nonfunctional rRNA decay; modified by the ubiquitin-like protein, Rub1p (2, 3, 4, 5) Also known as: CULC
Name Description	Regulator of Ty1 Transposition 1
Gene Product Alias	CULLIN 8

Chromosomal Location	ChrX:352108 to 349580 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.

	View Computational GO annotations for RTT101
Molecular Function
Manually curated	ubiquitin-protein ligase activity (IDA)
Biological Process
Manually curated	negative regulation of transposition, RNA-mediated (IMP) nonfunctional rRNA decay (IMP) regulation of mitosis (IMP) replication fork processing (IMP) ubiquitin-dependent protein catabolic process (IC)
Cellular Component
Manually curated	Cul8-RING ubiquitin ligase complex (IDA)
High-throughput	cytoplasm (IDA) nucleus (IDA)

Classical genetics
null	budding index: increased cell cycle progression in mid anaphase: delayed growth in exponential phase: decreased rate Rad53p modification: increased Mms22p-HA modification: decreased resistance to camptothecin: decreased resistance to methyl methanesulfonate: decreased resistance to hydroxyurea: decreased resistance to tellurite: decreased transposable element transposition: normal transposable element transposition: increased viable
Large-scale survey
null	budding index: increased cell cycle progression in G1 phase: decreased duration competitive fitness: decreased dessication resistance: decreased haploproficient heat sensitivity: increased mitotic recombination: increased oxidative stress resistance: decreased Rad52-YFP distribution: increased resistance to methyl methanesulfonate: decreased resistance to hydroxyurea: decreased resistance to caffeine: decreased resistance to cycloheximide: decreased resistance to camptothecin: decreased resistance to bleomycin: decreased resistance to sodium arsenite: decreased resistance to arsenite(3-): decreased resistance to tirapazamine: decreased resistance to tunicamycin: increased resistance to fluconazole: increased resistance to fenpropimorph: increased resistance to sodium selenide: decreased resistance to doxorubicin: decreased resistance to isopropyl (N-3-chloro-phenyl)-carbamate: decreased resistance to Azaserine: decreased resistance to thiabendazole: decreased toxin resistance: decreased transposable element transposition: increased vacuolar morphology: abnormal viable
overexpression	resistance to sirolimus: decreased
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	231 total interaction(s) for 147 unique genes/features.
Physical Interactions	Affinity Capture-MS: 14 Affinity Capture-RNA: 1 Affinity Capture-Western: 17 Biochemical Activity: 2 Reconstituted Complex: 3 Two-hybrid: 3
Genetic Interactions	Dosage Growth Defect: 2 Dosage Lethality: 1 Negative Genetic: 105 Phenotypic Enhancement: 1 Phenotypic Suppression: 6 Positive Genetic: 21 Synthetic Growth Defect: 43 Synthetic Lethality: 9 Synthetic Rescue: 3
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder | YeastRC Two-Hybrid

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrX:352108 to 349580 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.
Last Update	Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..2529 352108..349580 2011-02-03 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain CLIB215 S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FL100 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain YPS163 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000003583