RSC4/YKR008W Summary Help

Standard Name RSC4 1
Systematic Name YKR008W
Feature Type ORF, Verified
Description Component of the RSC chromatin remodeling complex; found in close proximity to nucleosomal DNA; displaced from the surface of nucleosomal DNA after chromatin remodeling; acetylated (K25) by Gcn5p, altering replication stress tolerance; contains tandem bromodomains that recognize histone H3 acetylated on K14 (H3K14ac) by Gcn5p (2, 3, 4, 5, 6, 7)
Name Description Remodel the Structure of Chromatin 8
Chromosomal Location
ChrXI:452201 to 454078 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All RSC4 GO evidence and references
  View Computational GO annotations for RSC4
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 2 genes
Resources
Classical genetics
conditional
null
repressible
Large-scale survey
conditional
null
overexpression
reduction of function
repressible
Resources
243 total interaction(s) for 138 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 107
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 10
  • Biochemical Activity: 1
  • Co-crystal Structure: 3
  • Co-purification: 3
  • PCA: 1
  • Protein-peptide: 1
  • Reconstituted Complex: 5
  • Two-hybrid: 1

Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Rescue: 1
  • Negative Genetic: 65
  • Phenotypic Enhancement: 1
  • Positive Genetic: 19
  • Synthetic Growth Defect: 1
  • Synthetic Lethality: 15
  • Synthetic Rescue: 6

Resources
Expression Summary
histogram
Resources
Length (a.a.) 625
Molecular Weight (Da) 72,291
Isoelectric Point (pI) 4.92
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXI:452201 to 454078 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 2011-02-03
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1878 452201..454078 2011-02-03 2011-02-03
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001716
References cited on this page View Complete Literature Guide for RSC4
1) Wysocki R, et al.  (1999) Mass-murdering: deletion of twenty-three ORFs from Saccharomyces cerevisiae chromosome XI reveals five genes essential for growth and three genes conferring detectable mutant phenotype. Gene 229(1-2):37-45
2) Sengupta SM, et al.  (2001) The interactions of yeast SWI/SNF and RSC with the nucleosome before and after chromatin remodeling. J Biol Chem 276(16):12636-44
3) Lorch Y, et al.  (1998) Activated RSC-nucleosome complex and persistently altered form of the nucleosome. Cell 94(1):29-34
4) Neely KE and Workman JL  (2002) The complexity of chromatin remodeling and its links to cancer. Biochim Biophys Acta 1603(1):19-29
5) Kasten M, et al.  (2004) Tandem bromodomains in the chromatin remodeler RSC recognize acetylated histone H3 Lys14. EMBO J 23(6):1348-59
6) VanDemark AP, et al.  (2007) Autoregulation of the rsc4 tandem bromodomain by gcn5 acetylation. Mol Cell 27(5):817-28
7) Charles GM, et al.  (2011) Site-specific acetylation mark on an essential chromatin-remodeling complex promotes resistance to replication stress. Proc Natl Acad Sci U S A 108(26):10620-5
8) Cairns BR, et al.  (1996) RSC, an essential, abundant chromatin-remodeling complex. Cell 87(7):1249-60