RPT3/YDR394W Summary Help

Standard Name RPT3 1
Systematic Name YDR394W
Alias YNT1 2 , YTA2 3
Feature Type ORF, Verified
Description ATPase of the 19S regulatory particle of the 26S proteasome; one of ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; substrate of N-acetyltransferase B (4, 5, 6, 7)
Name Description Regulatory Particle Triple-A protein, or Regulatory Particle Triphosphatase 1
Chromosomal Location
ChrIV:1261681 to 1262967 | ORF Map | GBrowse
Gene Ontology Annotations All RPT3 GO evidence and references
  View Computational GO annotations for RPT3
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 5 genes
Classical genetics
reduction of function
Large-scale survey
reduction of function
334 total interaction(s) for 153 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 153
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 20
  • Co-crystal Structure: 3
  • Co-fractionation: 5
  • Co-purification: 9
  • PCA: 4
  • Reconstituted Complex: 4
  • Two-hybrid: 20

Genetic Interactions
  • Negative Genetic: 66
  • Phenotypic Suppression: 2
  • Positive Genetic: 40
  • Synthetic Growth Defect: 4
  • Synthetic Rescue: 2

Expression Summary
Length (a.a.) 428
Molecular Weight (Da) 47,893
Isoelectric Point (pI) 5.27
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrIV:1261681 to 1262967 | ORF Map | GBrowse
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1287 1261681..1262967 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002802
References cited on this page View Complete Literature Guide for RPT3
1) Finley D, et al.  (1998) Unified nomenclature for subunits of the Saccharomyces cerevisiae proteasome regulatory particle. Trends Biochem Sci 23(7):244-5
2) Campbell CL, et al.  (1994) Mitochondrial morphological and functional defects in yeast caused by yme1 are suppressed by mutation of a 26S protease subunit homologue. Mol Biol Cell 5(8):899-905
3) Schnall R, et al.  (1994) Identification of a set of yeast genes coding for a novel family of putative ATPases with high similarity to constituents of the 26S protease complex. Yeast 10(9):1141-55
4) Glickman MH, et al.  (1998) The regulatory particle of the Saccharomyces cerevisiae proteasome. Mol Cell Biol 18(6):3149-62
5) Glickman MH, et al.  (1999) Functional analysis of the proteasome regulatory particle. Mol Biol Rep 26(1-2):21-8
6) Rubin DM, et al.  (1998) Active site mutants in the six regulatory particle ATPases reveal multiple roles for ATP in the proteasome. EMBO J 17(17):4909-19
7) Kimura Y, et al.  (2003) N-Terminal modifications of the 19S regulatory particle subunits of the yeast proteasome. Arch Biochem Biophys 409(2):341-8