RPO41/YFL036W Summary Help

Standard Name RPO41
Systematic Name YFL036W
Feature Type ORF, Verified
Description Mitochondrial RNA polymerase; single subunit enzyme similar to those of T3 and T7 bacteriophages; requires a specificity subunit encoded by MTF1 for promoter recognition; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation (1, 2, 3)
Name Description RNA POlymerase
Chromosomal Location
ChrVI:58782 to 62837 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All RPO41 GO evidence and references
  View Computational GO annotations for RPO41
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 2 genes
Resources
Classical genetics
conditional
null
reduction of function
Large-scale survey
null
unspecified
Resources
138 total interaction(s) for 118 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 13
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 1
  • Biochemical Activity: 2
  • Co-purification: 1
  • PCA: 1
  • Two-hybrid: 5

Genetic Interactions
  • Dosage Rescue: 7
  • Negative Genetic: 74
  • Phenotypic Enhancement: 1
  • Positive Genetic: 10
  • Synthetic Growth Defect: 10
  • Synthetic Lethality: 10
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 1,351
Molecular Weight (Da) 153,046
Isoelectric Point (pI) 9.55
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVI:58782 to 62837 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..4056 58782..62837 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001858
References cited on this page View Complete Literature Guide for RPO41
1) Greenleaf AL, et al.  (1986) Yeast RPO41 gene product is required for transcription and maintenance of the mitochondrial genome. Proc Natl Acad Sci U S A 83(10):3391-4
2) Tang GQ, et al.  (2011) Transcription factor-dependent DNA bending governs promoter recognition by the mitochondrial RNA polymerase. J Biol Chem 286(44):38805-13
3) Velazquez G, et al.  (2012) Conservation of promoter melting mechanisms in divergent regions of the single-subunit RNA polymerases. Biochemistry 51(18):3901-10