RPN9/YDR427W Summary Help

Standard Name RPN9 1
Systematic Name YDR427W
Alias NAS7 2
Feature Type ORF, Verified
Description Non-ATPase regulatory subunit of the 26S proteasome; similar to putative proteasomal subunits in other species; null mutant is temperature sensitive and exhibits cell cycle and proteasome assembly defects; protein abundance increases in response to DNA replication stress; relocalizes to the cytosol in response to hypoxia (3, 4, 5, 6)
Name Description Regulatory Particle Non-ATPase 1
Chromosomal Location
ChrIV:1322205 to 1323386 | ORF Map | GBrowse
Gene Ontology Annotations All RPN9 GO evidence and references
  View Computational GO annotations for RPN9
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 6 genes
Classical genetics
Large-scale survey
186 total interaction(s) for 56 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 142
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 21
  • Co-purification: 3
  • PCA: 3
  • Reconstituted Complex: 3
  • Two-hybrid: 8

Genetic Interactions
  • Dosage Lethality: 1
  • Phenotypic Suppression: 1
  • Synthetic Haploinsufficiency: 1
  • Synthetic Rescue: 2

Expression Summary
Length (a.a.) 393
Molecular Weight (Da) 45,782
Isoelectric Point (pI) 5.56
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrIV:1322205 to 1323386 | ORF Map | GBrowse
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1182 1322205..1323386 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002835
References cited on this page View Complete Literature Guide for RPN9
1) Finley D, et al.  (1998) Unified nomenclature for subunits of the Saccharomyces cerevisiae proteasome regulatory particle. Trends Biochem Sci 23(7):244-5
2) Inai Y and Nishikimi M  (2002) Increased degradation of oxidized proteins in yeast defective in 26 S proteasome assembly. Arch Biochem Biophys 404(2):279-84
3) Glickman MH, et al.  (1998) The regulatory particle of the Saccharomyces cerevisiae proteasome. Mol Cell Biol 18(6):3149-62
4) Takeuchi J and Toh-e A  (2001) Genetic dissection of the yeast 26S proteasome: cell cycle defects caused by the Deltarpn9 mutation. Biochimie 83(3-4):333-40
5) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
6) Ghosh Dastidar R, et al.  (2012) The nuclear localization of SWI/SNF proteins is subjected to oxygen regulation. Cell Biosci 2(1):30