RPN4/YDL020C Summary Help

Standard Name RPN4 1
Systematic Name YDL020C
Alias SON1 2 , UFD5 3
Feature Type ORF, Verified
Description Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress (4, 5, 6, 7, 8)
Name Description Regulatory Particle Non-ATPase 1
Chromosomal Location
ChrIV:416708 to 415113 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All RPN4 GO evidence and references
  View Computational GO annotations for RPN4
Molecular Function
Manually curated
High-throughput
Biological Process
Manually curated
High-throughput
Cellular Component
Manually curated
High-throughput
Targets 113 genes
Regulators 13 genes
Resources
Classical genetics
null
Large-scale survey
null
overexpression
Resources
916 total interaction(s) for 515 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 6
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 13
  • Biochemical Activity: 4
  • Co-fractionation: 1
  • Co-purification: 1
  • Reconstituted Complex: 3
  • Two-hybrid: 3

Genetic Interactions
  • Dosage Growth Defect: 7
  • Dosage Rescue: 4
  • Negative Genetic: 418
  • Phenotypic Enhancement: 36
  • Phenotypic Suppression: 45
  • Positive Genetic: 54
  • Synthetic Growth Defect: 236
  • Synthetic Lethality: 72
  • Synthetic Rescue: 11

Resources
Expression Summary
histogram
Resources
Length (a.a.) 531
Molecular Weight (Da) 60,152
Isoelectric Point (pI) 5.14
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIV:416708 to 415113 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1596 416708..415113 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002178
References cited on this page View Complete Literature Guide for RPN4
1) Finley D, et al.  (1998) Unified nomenclature for subunits of the Saccharomyces cerevisiae proteasome regulatory particle. Trends Biochem Sci 23(7):244-5
2) Nelson MK, et al.  (1993) Extragenic suppressors of mutations in the cytoplasmic C terminus of SEC63 define five genes in Saccharomyces cerevisiae. Genetics 134(1):159-73
3) Johnson ES, et al.  (1995) A proteolytic pathway that recognizes ubiquitin as a degradation signal. J Biol Chem 270(29):17442-56
4) Ng DT, et al.  (2000) The unfolded protein response regulates multiple aspects of secretory and membrane protein biogenesis and endoplasmic reticulum quality control. J Cell Biol 150(1):77-88
5) Xie Y and Varshavsky A  (2001) RPN4 is a ligand, substrate, and transcriptional regulator of the 26S proteasome: a negative feedback circuit. Proc Natl Acad Sci U S A 98(6):3056-61
6) Mannhaupt G, et al.  (1999) Rpn4p acts as a transcription factor by binding to PACE, a nonamer box found upstream of 26S proteasomal and other genes in yeast. FEBS Lett 450(1-2):27-34
7) Owsianik G, et al.  (2002) Control of 26S proteasome expression by transcription factors regulating multidrug resistance in Saccharomyces cerevisiae. Mol Microbiol 43(5):1295-308
8) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
9) Zhu C, et al.  (2009) High-resolution DNA-binding specificity analysis of yeast transcription factors. Genome Res 19(4):556-66
10) Harbison CT, et al.  (2004) Transcriptional regulatory code of a eukaryotic genome. Nature 431(7004):99-104
11) Badis G, et al.  (2008) A library of yeast transcription factor motifs reveals a widespread function for Rsc3 in targeting nucleosome exclusion at promoters. Mol Cell 32(6):878-87