RPN13/YLR421C Summary Help

Standard Name RPN13 1
Systematic Name YLR421C
Feature Type ORF, Verified
Description Subunit of the 19S regulatory particle of the 26S proteasome lid; acts as a ubiquitin receptor for the proteasome; null mutants accumulate ubiquitinated Gcn4p and display decreased 26S proteasome stability; protein abundance increases in response to DNA replication stress (1, 2, 3, 4)
Name Description Regulatory Particle Non-ATPase 5
Chromosomal Location
ChrXII:965560 to 965090 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All RPN13 GO evidence and references
  View Computational GO annotations for RPN13
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 123 genes
Resources
Classical genetics
null
Large-scale survey
null
Resources
153 total interaction(s) for 83 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 72
  • Affinity Capture-RNA: 5
  • Affinity Capture-Western: 7
  • Co-purification: 1
  • PCA: 1
  • Reconstituted Complex: 3
  • Two-hybrid: 3

Genetic Interactions
  • Negative Genetic: 51
  • Phenotypic Enhancement: 1
  • Positive Genetic: 7
  • Synthetic Growth Defect: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 156
Molecular Weight (Da) 17,902
Isoelectric Point (pI) 4.28
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXII:965560 to 965090 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..471 965560..965090 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004413
References cited on this page View Complete Literature Guide for RPN13
1) Verma R, et al.  (2000) Proteasomal proteomics: identification of nucleotide-sensitive proteasome-interacting proteins by mass spectrometric analysis of affinity-purified proteasomes. Mol Biol Cell 11(10):3425-39
2) Seong KM, et al.  (2007) Rpn13p and Rpn14p are involved in the recognition of ubiquitinated Gcn4p by the 26S proteasome. FEBS Lett 581(13):2567-73
3) Husnjak K, et al.  (2008) Proteasome subunit Rpn13 is a novel ubiquitin receptor. Nature 453(7194):481-488
4) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
5) Finley D, et al.  (1998) Unified nomenclature for subunits of the Saccharomyces cerevisiae proteasome regulatory particle. Trends Biochem Sci 23(7):244-5