Standard Name	RPN10 1
Systematic Name	YHR200W
Alias	MCB1 2 , SUN1 3
Feature Type	ORF, Verified
Description	Non-ATPase base subunit of the 19S regulatory particle (RP) of the 26S proteasome; N-terminus plays a role in maintaining the structural integrity of the RP; binds selectively to polyubiquitin chains; homolog of the mammalian S5a protein (1, 4, 5, 6)
Name Description	Regulatory Particle Non-ATPase 1

Chromosomal Location	ChrVIII:499079 to 499885 | ORF Map | GBrowse

	View Computational GO annotations for RPN10
Molecular Function
Manually curated	polyubiquitin binding (IDA) structural molecule activity (IMP)
Biological Process
Manually curated	ubiquitin-dependent protein catabolic process (IMP)
Cellular Component
Manually curated	proteasome complex (IMP) proteasome regulatory particle, base subcomplex (IDA, IPI)

Classical genetics
null	resistance to bleomycin A2: decreased resistance to canavanine: decreased resistance to L-azetidine-2-carboxylic acid: decreased resistance to bortezomib: decreased viable
Large-scale survey
null	bud morphology: abnormal polyphosphate accumulation: abnormal competitive fitness: decreased dessication resistance: decreased haploinsufficient resistance to fluconazole: increased resistance to bleomycin: increased resistance to camptothecin: decreased resistance to cycloheximide: decreased resistance to BPS: decreased resistance to fenpropimorph: increased resistance to calcium dichloride: decreased resistance to benzo[a]pyrene: decreased sporulation: decreased sporulation efficiency: decreased vacuolar morphology: abnormal vegetative growth: decreased rate viable
overexpression	vegetative growth: decreased rate
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	1197 total interaction(s) for 647 unique genes/features.
Physical Interactions	Affinity Capture-MS: 522 Affinity Capture-RNA: 3 Affinity Capture-Western: 42 Biochemical Activity: 2 Co-crystal Structure: 1 Co-fractionation: 5 Co-purification: 11 Far Western: 2 Reconstituted Complex: 15 Two-hybrid: 2
Genetic Interactions	Dosage Growth Defect: 2 Dosage Rescue: 2 Negative Genetic: 288 Phenotypic Enhancement: 5 Phenotypic Suppression: 4 Positive Genetic: 73 Synthetic Growth Defect: 126 Synthetic Lethality: 49 Synthetic Rescue: 43
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | AspGD Homologs | CGD Homologs | CandidaDB Homologs | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrVIII:499079 to 499885 | ORF Map | GBrowse
Last Update	Coordinates: 2005-11-07 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..807 499079..499885 2005-11-07 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI1631 S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain CLIB215 S.c. Strain CLIB324 S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FL100 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain T73 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain YPS163 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000001243