Standard Name	RPH1
Systematic Name	YER169W
Feature Type	ORF, Verified
Description	JmjC domain-containing histone demethylase; specifically demethylates H3K36 tri- and dimethyl modification states; associates with actively transcribed (RNAP II) regions in vivo and specifically targets H3K36 in its trimethylation state as its substrate; transcriptional repressor of PHR1; Rph1p phosphorylation during DNA damage is under control of the MEC1-RAD53 pathway; target of stess-induced hormesis; RPH1 has a paralog, GIS1, that arose from the whole genome duplication (1, 2, 3, 4, 5, 6, 7)
Name Description	Regulator of PHR1 1
Gene Product Alias	KDM4 8

Chromosomal Location	ChrV:523369 to 525759 | ORF Map | GBrowse

	View Computational GO annotations for RPH1
Molecular Function
Manually curated	histone demethylase activity (H3-K36 specific) (IDA, IMP) histone demethylase activity (H3-K9 specific) (IDA) RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription (IDA, IMP) sequence-specific DNA binding (IDA, IMP)
High-throughput	sequence-specific DNA binding (IDA)
Biological Process
Manually curated	histone demethylation (IDA, IMP) negative regulation of transcription from RNA polymerase II promoter (IDA)
Cellular Component
Manually curated	nucleus (IC)

Binding motifs	Predicted RPH1 Binding Site Locations
	CCCCTTAAGG 1 [Search intergenic regions for CCCCTTAAGG] CCCCTWA 9 [Search intergenic regions for CCCCTWA] TTAGGGG 10 [Search intergenic regions for TTAGGGG]
Resources	ScerTF | UniProbe | YEASTRACT | YeTFaSCo

Classical genetics
null	chronological lifespan: decreased histone H3K36me3 modification: increased resistance to sirolimus: increased
Large-scale survey
null	auxotrophy competitive fitness: decreased vegetative growth: decreased rate viable
overexpression	vegetative growth: decreased rate
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	38 total interaction(s) for 34 unique genes/features.
Physical Interactions	Affinity Capture-MS: 2 Affinity Capture-RNA: 1 Affinity Capture-Western: 1 Biochemical Activity: 2 Protein-peptide: 2
Genetic Interactions	Dosage Rescue: 2 Negative Genetic: 18 Phenotypic Enhancement: 1 Positive Genetic: 7 Synthetic Growth Defect: 2
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder | YeastRC Two-Hybrid

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrV:523369 to 525759 | ORF Map | GBrowse
Last Update	Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..2391 523369..525759 2011-02-03 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CEN.PK113-7D S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FL100 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain T73 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain YJM789 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000000971