RPH1/YER169W Summary Help

Standard Name RPH1 1
Systematic Name YER169W
Feature Type ORF, Verified
Description JmjC domain-containing histone demethylase; specifically demethylates H3K36 tri- and dimethyl modification states; associates with actively transcribed (RNAP II) regions in vivo and specifically targets H3K36 in its trimethylation state as its substrate; transcriptional repressor of PHR1; Rph1p phosphorylation during DNA damage is under control of the MEC1-RAD53 pathway; target of stess-induced hormesis; RPH1 has a paralog, GIS1, that arose from the whole genome duplication (1, 2, 3, 4, 5, 6, 7)
Name Description Regulator of PHR1 1
Gene Product Alias KDM4 8
Chromosomal Location
ChrV:523369 to 525759 | ORF Map | GBrowse
Gene Ontology Annotations All RPH1 GO evidence and references
  View Computational GO annotations for RPH1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Targets 34 genes
Regulators 2 genes
Classical genetics
Large-scale survey
44 total interaction(s) for 39 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 3
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 2
  • Biochemical Activity: 2
  • Co-localization: 4
  • Protein-peptide: 2

Genetic Interactions
  • Dosage Rescue: 2
  • Negative Genetic: 18
  • Phenotypic Enhancement: 1
  • Positive Genetic: 7
  • Synthetic Growth Defect: 2

Expression Summary
Length (a.a.) 796
Molecular Weight (Da) 90,211
Isoelectric Point (pI) 9.84
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrV:523369 to 525759 | ORF Map | GBrowse
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..2391 523369..525759 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000971
References cited on this page View Complete Literature Guide for RPH1
1) Jang YK, et al.  (1999) RPH1 and GIS1 are damage-responsive repressors of PHR1. Mol Cell Biol 19(11):7630-8
2) Kim EM, et al.  (2002) Phosphorylation of Rph1, a damage-responsive repressor of PHR1 in Saccharomyces cerevisiae, is dependent upon Rad53 kinase. Nucleic Acids Res 30(3):643-8
3) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
4) Tu S, et al.  (2007) Identification of histone demethylases in Saccharomyces cerevisiae. J Biol Chem 282(19):14262-71
5) Klose RJ, et al.  (2007) Demethylation of histone H3K36 and H3K9 by Rph1: a vestige of an H3K9 methylation system in Saccharomyces cerevisiae? Mol Cell Biol 27(11):3951-61
6) Kwon DW and Ahn SH  (2011) Role of yeast JmjC-domain containing histone demethylases in actively transcribed regions. Biochem Biophys Res Commun 410(3):614-9
7) Schroeder EA, et al.  (2013) Epigenetic silencing mediates mitochondria stress-induced longevity. Cell Metab 17(6):954-64
8) Allis CD, et al.  (2007) New nomenclature for chromatin-modifying enzymes. Cell 131(4):633-6
9) Badis G, et al.  (2008) A library of yeast transcription factor motifs reveals a widespread function for Rsc3 in targeting nucleosome exclusion at promoters. Mol Cell 32(6):878-87
10) Zhu C, et al.  (2009) High-resolution DNA-binding specificity analysis of yeast transcription factors. Genome Res 19(4):556-66