RPF2/YKR081C Summary Help

Standard Name RPF2
Systematic Name YKR081C
Feature Type ORF, Verified
Description Essential protein involved in rRNA maturation and ribosomal assembly; involved in the processing of pre-rRNA and the assembly of the 60S ribosomal subunit; interacts with ribosomal protein L11; localizes predominantly to the nucleolus; constituent of 66S pre-ribosomal particles (1, 2)
Chromosomal Location
ChrXI:592540 to 591506 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All RPF2 GO evidence and references
  View Computational GO annotations for RPF2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 3 genes
Resources
Classical genetics
null
Large-scale survey
conditional
null
overexpression
reduction of function
Resources
523 total interaction(s) for 282 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 407
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 95
  • Co-fractionation: 1
  • Co-purification: 5
  • Far Western: 4
  • PCA: 1
  • Two-hybrid: 5

Genetic Interactions
  • Dosage Rescue: 1
  • Positive Genetic: 1
  • Synthetic Lethality: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 344
Molecular Weight (Da) 39,598
Isoelectric Point (pI) 9.75
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXI:592540 to 591506 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1035 592540..591506 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001789
References cited on this page View Complete Literature Guide for RPF2
1) Morita D, et al.  (2002) Rpf2p, an evolutionarily conserved protein, interacts with ribosomal protein L11 and is essential for the processing of 27 SB Pre-rRNA to 25 S rRNA and the 60 S ribosomal subunit assembly in Saccharomyces cerevisiae. J Biol Chem 277(32):28780-6
2) Horsey EW, et al.  (2004) Role of the yeast Rrp1 protein in the dynamics of pre-ribosome maturation. RNA 10(5):813-27