RPF1/YHR088W Summary Help

Standard Name RPF1
Systematic Name YHR088W
Feature Type ORF, Verified
Description Protein involved in assembly and export of the large ribosomal subunit; nucleolar protein; constituent of 66S pre-ribosomal particles; contains a sigma(70)-like motif, which is thought to bind RNA (1, 2, 3, 4)
Chromosomal Location
ChrVIII:281495 to 282382 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All RPF1 GO evidence and references
  View Computational GO annotations for RPF1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 8 genes
Resources
Classical genetics
conditional
null
Large-scale survey
conditional
null
Resources
121 total interaction(s) for 84 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 83
  • Affinity Capture-RNA: 2
  • Biochemical Activity: 1
  • Co-localization: 1
  • Reconstituted Complex: 6
  • Two-hybrid: 1

Genetic Interactions
  • Dosage Rescue: 1
  • Negative Genetic: 20
  • Phenotypic Suppression: 1
  • Synthetic Growth Defect: 1
  • Synthetic Lethality: 4

Resources
Expression Summary
histogram
Resources
Length (a.a.) 295
Molecular Weight (Da) 35,121
Isoelectric Point (pI) 10.28
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVIII:281495 to 282382 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..888 281495..282382 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001130
References cited on this page View Complete Literature Guide for RPF1
1) Wehner KA and Baserga SJ  (2002) The sigma(70)-like motif: a eukaryotic RNA binding domain unique to a superfamily of proteins required for ribosome biogenesis. Mol Cell 9(2):329-39
2) Bogengruber E, et al.  (2003) Functional analysis in yeast of the Brix protein superfamily involved in the biogenesis of ribosomes. FEMS Yeast Res 3(1):35-43
3) Horsey EW, et al.  (2004) Role of the yeast Rrp1 protein in the dynamics of pre-ribosome maturation. RNA 10(5):813-27
4) Caesar S, et al.  (2006) Kap120 functions as a nuclear import receptor for ribosome assembly factor Rpf1 in yeast. Mol Cell Biol 26(8):3170-80