RPE1/YJL121C Summary Help

Standard Name RPE1 1
Systematic Name YJL121C
Alias EPI1 , POS18 1
Feature Type ORF, Verified
Description D-ribulose-5-phosphate 3-epimerase; catalyzes a reaction in the non-oxidative part of the pentose-phosphate pathway; mutants are sensitive to oxidative stress (1, 2 and see Summary Paragraph)
Name Description Ribulose 5-Phosphate Epimerase 1
Chromosomal Location
ChrX:191090 to 190374 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All RPE1 GO evidence and references
  View Computational GO annotations for RPE1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 4 genes
Classical genetics
Large-scale survey
70 total interaction(s) for 60 unique genes/features.
Physical Interactions
  • Affinity Capture-RNA: 3
  • Two-hybrid: 1

Genetic Interactions
  • Negative Genetic: 43
  • Positive Genetic: 8
  • Synthetic Growth Defect: 4
  • Synthetic Lethality: 11

Expression Summary
Length (a.a.) 238
Molecular Weight (Da) 25,967
Isoelectric Point (pI) 6.24
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrX:191090 to 190374 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..717 191090..190374 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003657

Rpe1p is a D-ribulose-5-phosphate 3-epimerase (EC: in the non-oxidative part of the pentose phosphate pathway (2). It is located in the cytosol (3). rpe1 null mutants are viable, but display no D-ribulose-5-phosphate 3-epimerase activity, and display increased sensitivity to hydrogen peroxide (1), reduced growth on yeast minimal medium (MM) (4), and no growth on media containing D-xylulose as the sole carbon source (2). The reduced fitness of rpe1 null mutants may be due to insufficient NADPH reserves for biosynthesis, since the pentose phosphate pathway is important for maintaining NADPH levels in the cell (4). Rpe1p is of industrial interest because deletion of RPE1 in a recombinant S. cerevisiae strain engineered to ferment xylose (the major pentose in lignocellulose) to ethanol results in the complete loss of ethanol production (5), and rpe1 null mutants display increased sensitivity to the fermentation inhibitor furfural, which is a byproduct of the fermentation of xylose to ethanol (6).

Rpe1p has similarity to the D-ribulose-5-phosphate 3-epimerases of Escherichia coli, Rhodospirillum rubrum, Alcaligenes eutrophus, and Solanum tuberosum (1), and has similarity to human RPE (OMIM; 7).

Last updated: 2006-01-13 Contact SGD

References cited on this page View Complete Literature Guide for RPE1
1) Juhnke H, et al.  (1996) Mutants that show increased sensitivity to hydrogen peroxide reveal an important role for the pentose phosphate pathway in protection of yeast against oxidative stress. Mol Gen Genet 252(4):456-64
2) Miosga T and Zimmermann FK  (1996) Cloning and characterization of the first two genes of the non-oxidative part of the Saccharomyces cerevisiae pentose-phosphate pathway. Curr Genet 30(5):404-9
3) Maaheimo H, et al.  (2001) Central carbon metabolism of Saccharomyces cerevisiae explored by biosynthetic fractional (13)C labeling of common amino acids. Eur J Biochem 268(8):2464-79
4) Blank LM, et al.  (2005) Large-scale 13C-flux analysis reveals mechanistic principles of metabolic network robustness to null mutations in yeast. Genome Biol 6(6):R49
5) Eliasson A, et al.  (2000) Xylulose fermentation by mutant and wild-type strains of Zygosaccharomyces and Saccharomyces cerevisiae. Appl Microbiol Biotechnol 53(4):376-82
6) Gorsich SW, et al.  (2006) Tolerance to furfural-induced stress is associated with pentose phosphate pathway genes ZWF1, GND1, RPE1, and TKL1 in Saccharomyces cerevisiae. Appl Microbiol Biotechnol 71(3):339-49
7) Stanchi F, et al.  (2001) Characterization of 16 novel human genes showing high similarity to yeast sequences. Yeast 18(1):69-80