Standard Name	ROX3
Systematic Name	YBL093C
Alias	NUT3 1 , SSN7 , MED19 2
Feature Type	ORF, Verified
Description	Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme (3, 4)
Name Description	Repressor Of hypoXic genes

Chromosomal Location	ChrII:44918 to 44256 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.

	View Computational GO annotations for ROX3
Molecular Function
Manually curated	RNA polymerase II transcription factor recruiting transcription factor activity (IMP)
Biological Process
Manually curated	negative regulation of transcription from RNA polymerase II promoter (IMP) positive regulation of transcription from RNA polymerase II promoter (IMP) RNA polymerase II transcriptional preinitiation complex assembly (IMP) transcriptional open complex formation at RNA polymerase II promoter (IMP)
Cellular Component
Manually curated	core mediator complex (IDA) nucleus (IDA)

Classical genetics
null	oxidative stress resistance: decreased resistance to 2-deoxy-D-glucose: increased resistance to hydroxyurea: decreased
Large-scale survey
conditional	resistance to methyl methanesulfonate: decreased
null	acid pH resistance: decreased cell size: increased competitive fitness: decreased fermentative growth: decreased rate filamentous growth: increased haploinsufficient heat sensitivity: increased lipid particle morphology: abnormal resistance to tirapazamine: decreased resistance to CTBT: decreased resistance to hydroxyurea: decreased resistance to neomycin: decreased resistance to wortmannin: decreased resistance to (S)-lactic acid: decreased resistance to acetic acid: decreased resistance to hydrogen chloride: decreased resistance to pyrrolidine dithiocarbamate: decreased resistance to boric acid: decreased resistance to calcium dichloride: decreased resistance to cycloheximide: decreased resistance to L-1,4-dithiothreitol: decreased resistance to ethanol: decreased resistance to hygromycin B: decreased resistance to sirolimus: decreased resistance to sorbate: decreased resistance to doxorubicin: decreased resistance to tunicamycin: decreased respiratory growth: absent telomere length: decreased toxin resistance: decreased utilization of carbon source: decreased utilization of nitrogen source: absent vegetative growth: decreased rate viable
overexpression	silencing: abnormal
Resources	PROPHECY | PhenoM | SCMD | ScreenTroll | Yeast Fitness Database

	124 total interaction(s) for 64 unique genes/features.
Physical Interactions	Affinity Capture-MS: 67 Affinity Capture-RNA: 1 Affinity Capture-Western: 12 Co-purification: 5 PCA: 1 Two-hybrid: 3
Genetic Interactions	Dosage Growth Defect: 4 Dosage Lethality: 1 Dosage Rescue: 3 Negative Genetic: 15 Phenotypic Enhancement: 1 Phenotypic Suppression: 3 Positive Genetic: 6 Synthetic Rescue: 2
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder | YeastRC Two-Hybrid

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | Organelle DB (UMich) | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrII:44918 to 44256 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.
Last Update	Coordinates: 2011-02-03 | Sequence: 1997-01-28
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..663 44918..44256 2011-02-03 1997-01-28
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI1631 S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain CLIB215 S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FL100 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain M22 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain YPS163 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000000189