ROX1/YPR065W Summary

ROX1/YPR065W Summary

Standard Name	ROX1 1
Systematic Name	YPR065W
Alias	REO1 2 , 3
Feature Type	ORF, Verified
Description	Heme-dependent repressor of hypoxic genes; mediates aerobic transcriptional repression of hypoxia induced genes such as COX5b and CYC7; repressor function regulated through decreased promoter occupancy in response to oxidative stress; contains an HMG domain that is responsible for DNA bending activity; involved in the hyperosmotic stress resistance (4, 5, 6, 7, 8, 9)
Name Description	Regulation by OXygen 1

Chromosomal Location	ChrXVI:679693 to 680799 \| ORF Map \| GBrowse

Gene Ontology Annotations All ROX1 GO evidence and references

	View Computational GO annotations for ROX1
Molecular Function
Manually curated	DNA binding, bending (IDA) RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription (IMP) RNA polymerase II transcription factor recruiting transcription factor activity (IMP) sequence-specific DNA binding (IDA)
High-throughput	sequence-specific DNA binding (IDA)
Biological Process
Manually curated	negative regulation of transcription from RNA polymerase II promoter (IGI, IMP) negative regulation of transcription from RNA polymerase II promoter in response to stress (IMP)
Cellular Component
Manually curated	nuclear chromatin (IC)

Regulatory Role

Binding motifs	Predicted ROX1 Binding Site Locations
	ATTGT 10 [Search intergenic regions for ATTGT] YYYATTGTTCTC 11 [Search intergenic regions for YYYATTGTTCTC]

Resources	ScerTF \| YEASTRACT \| YeTFaSCo

Mutant phenotypes All ROX1 Phenotype evidence and references

Classical genetics
null	metal resistance: decreased resistance to 4-nitroquinoline-1-oxide: increased resistance to ferrozine(2-): decreased resistance to cobalt chloride hexahydrate: decreased resistance to fenpropimorph: decreased resistance to caffeine: decreased resistance to sirolimus: decreased
Large-scale survey
null	anaerobic growth: decreased cell cycle progression in G1 phase: delayed competitive fitness: decreased metal resistance: decreased resistance to 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid: decreased stress resistance: decreased viable
overexpression	vegetative growth: decreased vegetative growth: decreased rate
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All ROX1 Interaction evidence and references

	83 total interaction(s) for 77 unique genes/features.
Physical Interactions	Affinity Capture-MS: 2 Affinity Capture-RNA: 1 PCA: 1
Genetic Interactions	Dosage Lethality: 2 Negative Genetic: 58 Phenotypic Enhancement: 6 Phenotypic Suppression: 4 Positive Genetic: 8 Synthetic Growth Defect: 1
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All ROX1 Protein evidence and references

Localization	YeastRC \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrXVI:679693 to 680799 | |

SGD ORF map

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..1107	679693..680799	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000006269

References cited on this page View Complete Literature Guide for ROX1

1)	Lowry CV and Zitomer RS (1984) Oxygen regulation of anaerobic and aerobic genes mediated by a common factor in yeast. Proc Natl Acad Sci U S A 81(19):6129-33
2)	Kwast KE, et al. (1997) REO1 and ROX1 are alleles of the same gene which encodes a transcriptional repressor of hypoxic genes in Saccharomyces cerevisiae. Curr Genet 32(6):377-83
3)	Trueblood CE and Poyton RO (1988) Identification of REO1, a gene involved in negative regulation of COX5b and ANB1 in aerobically grown Saccharomyces cerevisiae. Genetics 120(3):671-80
4)	Deckert J, et al. (1995) Multiple elements and auto-repression regulate Rox1, a repressor of hypoxic genes in Saccharomyces cerevisiae. Genetics 139(3):1149-58
5)	Keng T (1992) HAP1 and ROX1 form a regulatory pathway in the repression of HEM13 transcription in Saccharomyces cerevisiae. Mol Cell Biol 12(6):2616-23
6)	Deckert J, et al. (1995) Mutational analysis of Rox1, a DNA-bending repressor of hypoxic genes in Saccharomyces cerevisiae. Mol Cell Biol 15(11):6109-17
7)	Ter Linde JJ and Steensma HY (2002) A microarray-assisted screen for potential Hap1 and Rox1 target genes in Saccharomyces cerevisiae. Yeast 19(10):825-40
8)	Montanes FM, et al. (2011) Repression of ergosterol biosynthesis is essential for stress resistance and is mediated by the Hog1 MAP kinase and the Mot3 and Rox1 transcription factors. Mol Microbiol 79(4):1008-23
9)	Liu J and Barrientos A (2012) Transcriptional Regulation of Yeast OXPHOS Hypoxic Genes by Oxidative Stress. Antioxid Redox Signal ()
10)	Badis G, et al. (2008) A library of yeast transcription factor motifs reveals a widespread function for Rsc3 in targeting nucleosome exclusion at promoters. Mol Cell 32(6):878-87
11)	Balasubramanian B, et al. (1993) The Rox1 repressor of the Saccharomyces cerevisiae hypoxic genes is a specific DNA-binding protein with a high-mobility-group motif. Mol Cell Biol 13(10):6071-8