RNQ1/YCL028W Summary Help

Standard Name RNQ1 1
Systematic Name YCL028W
Feature Type ORF, Verified
Description [PIN(+)] prion; an infectious protein conformation that is generally an ordered protein aggregate (1, 2 and see Summary Paragraph)
Also known as: [PIN(+)]
Name Description Rich in asparagine (N) and glutamine (Q) 1
Chromosomal Location
ChrIII:70150 to 71367 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All RNQ1 GO evidence and references
  View Computational GO annotations for RNQ1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 2 genes
Resources
Classical genetics
null
overexpression
Large-scale survey
null
overexpression
Resources
114 total interaction(s) for 87 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 30
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 33
  • Co-crystal Structure: 2
  • Protein-peptide: 1
  • Reconstituted Complex: 7
  • Two-hybrid: 19

Genetic Interactions
  • Dosage Rescue: 14
  • Phenotypic Enhancement: 1
  • Phenotypic Suppression: 6

Resources
Expression Summary
histogram
Resources
Length (a.a.) 405
Molecular Weight (Da) 42,580
Isoelectric Point (pI) 6.63
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIII:70150 to 71367 | ORF Map | GBrowse
This feature contains embedded feature(s): YCLX06C
SGD ORF map
Last Update Coordinates: 2000-09-13 | Sequence: 2000-09-13
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1218 70150..71367 2000-09-13 2000-09-13
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000533
SUMMARY PARAGRAPH for RNQ1

The [PSI+] prion determinant causes nonsense suppressor phenotype due to a reduced function of the translation termination factor Sup35p (eRF3) polymerized into amyloid fibrils. Prion state of the Rnq1 protein, [PIN+], is required for the [PSI+] de novo generation, but not propagation. Yeast [psi-] [PIN+] cells overproducing Sup35p can exhibit nonsense suppression without generation of a stable [PSI+]. In such cells most of Sup35p is present in amyloid polymers, though remaining Sup35p monomer is sufficient for normal translation termination. Presence of these polymers strictly depends on [PIN+], suggesting that their maintenance relies on efficient generation de novo, rather than inheritance. Sup35p polymers contain Rnq1p, confirming that Rnq1p polymers seed Sup35p polymerization (3).

Last updated: 2005-03-15 Contact SGD

References cited on this page View Complete Literature Guide for RNQ1
1) Sondheimer N and Lindquist S  (2000) Rnq1: an epigenetic modifier of protein function in yeast. Mol Cell 5(1):163-72
2) Derkatch IL, et al.  (2004) Effects of Q/N-rich, polyQ, and non-polyQ amyloids on the de novo formation of the [PSI+] prion in yeast and aggregation of Sup35 in vitro. Proc Natl Acad Sci U S A 101(35):12934-9
3) Salnikova AB, et al.  (2005) Nonsense suppression in yeast cells overproducing Sup35 (eRF3) is caused by its non-heritable amyloids. J Biol Chem 280(10):8808-12