RNH201/YNL072W Summary Help

Standard Name RNH201 1
Systematic Name YNL072W
Alias RNH35 2
Feature Type ORF, Verified
Description Ribonuclease H2 catalytic subunit; removes RNA primers during Okazaki fragment synthesis and errant ribonucleotides misincorporated during DNA replication; role in ribonucleotide excision repair; homolog of RNAse HI; related to human AGS4 which causes Aicardi-Goutieres syndrome (1, 2, 3, 4, 5)
Name Description RNase H 2
Gene Product Alias Rnh2A
Chromosomal Location
ChrXIV:490317 to 491240 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All RNH201 GO evidence and references
  View Computational GO annotations for RNH201
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 4 genes
Resources
Classical genetics
null
Large-scale survey
null
Resources
101 total interaction(s) for 66 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 3
  • Affinity Capture-RNA: 3
  • Reconstituted Complex: 2

Genetic Interactions
  • Dosage Growth Defect: 1
  • Negative Genetic: 29
  • Phenotypic Enhancement: 13
  • Phenotypic Suppression: 4
  • Positive Genetic: 12
  • Synthetic Growth Defect: 20
  • Synthetic Lethality: 12
  • Synthetic Rescue: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 307
Molecular Weight (Da) 34,875
Isoelectric Point (pI) 9.62
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXIV:490317 to 491240 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..924 490317..491240 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005016
References cited on this page View Complete Literature Guide for RNH201
1) McElhinny SA, et al.  (2010) Genome instability due to ribonucleotide incorporation into DNA. Nat Chem Biol 6(10):774-81
2) Frank P, et al.  (1998) Yeast RNase H(35) is the counterpart of the mammalian RNase HI, and is evolutionarily related to prokaryotic RNase HII. FEBS Lett 421(1):23-6
3) Qiu J, et al.  (1999) Saccharomyces cerevisiae RNase H(35) functions in RNA primer removal during lagging-strand DNA synthesis, most efficiently in cooperation with Rad27 nuclease. Mol Cell Biol 19(12):8361-71
4) Crow YJ, et al.  (2006) Mutations in genes encoding ribonuclease H2 subunits cause Aicardi-Goutieres syndrome and mimic congenital viral brain infection. Nat Genet 38(8):910-6
5) Sparks JL, et al.  (2012) RNase H2-initiated ribonucleotide excision repair. Mol Cell 47(6):980-6