RNA14/YMR061W Summary Help

Standard Name RNA14
Systematic Name YMR061W
Feature Type ORF, Verified
Description Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; bridges interaction between Rna15p and Hrp1p in the CF I complex; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping and maintenance of genome stability; relocalizes to the cytosol in response to hypoxia (1, 2, 3, 4, 5, 6, 7)
Name Description RNA synthesis
Chromosomal Location
ChrXIII:392755 to 394788 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All RNA14 GO evidence and references
  View Computational GO annotations for RNA14
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 3 genes
Resources
Classical genetics
conditional
Large-scale survey
conditional
null
overexpression
reduction of function
Resources
202 total interaction(s) for 99 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 76
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 16
  • Co-crystal Structure: 1
  • Co-fractionation: 2
  • Co-purification: 1
  • Far Western: 1
  • Reconstituted Complex: 11
  • Two-hybrid: 14

Genetic Interactions
  • Dosage Rescue: 4
  • Negative Genetic: 10
  • Phenotypic Enhancement: 1
  • Phenotypic Suppression: 3
  • Positive Genetic: 8
  • Synthetic Growth Defect: 26
  • Synthetic Lethality: 19
  • Synthetic Rescue: 6

Resources
Expression Summary
histogram
Resources
Length (a.a.) 677
Molecular Weight (Da) 79,960
Isoelectric Point (pI) 5.56
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXIII:392755 to 394788 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2034 392755..394788 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004665
References cited on this page View Complete Literature Guide for RNA14
1) Gross S and Moore C  (2001) Five subunits are required for reconstitution of the cleavage and polyadenylation activities of Saccharomyces cerevisiae cleavage factor I. Proc Natl Acad Sci U S A 98(11):6080-5
2) Gross S and Moore CL  (2001) Rna15 interaction with the A-rich yeast polyadenylation signal is an essential step in mRNA 3'-end formation. Mol Cell Biol 21(23):8045-55
3) Tous C, et al.  (2011) A novel assay identifies transcript elongation roles for the Nup84 complex and RNA processing factors. EMBO J 30(10):1953-64
4) Medler S, et al.  (2011) Evidence for a complex of transcription factor IIB with poly(A) polymerase and cleavage factor 1 subunits required for gene looping. J Biol Chem 286(39):33709-18
5) Gordon J, et al.  (2011) Reconstitution of CF IA from overexpressed subunits reveals stoichiometry and provides insights into molecular topology. Biochemistry 50(47):10203-14
6) Ghosh Dastidar R, et al.  (2012) The nuclear localization of SWI/SNF proteins is subjected to oxygen regulation. Cell Biosci 2(1):30
7) Gaillard H and Aguilera A  (2014) Cleavage Factor I Links Transcription Termination to DNA Damage Response and Genome Integrity Maintenance in Saccharomyces cerevisiae. PLoS Genet 10(3):e1004203