RMI1/YPL024W Summary Help

Standard Name RMI1 1, 2
Systematic Name YPL024W
Alias NCE4
Feature Type ORF, Verified
Description Subunit of the RecQ (Sgs1p) - Topo III (Top3p) complex; stimulates superhelical relaxing, DNA catenation/decatenation and ssDNA binding activities of Top3p; involved in response to DNA damage; functions in S phase-mediated cohesion establishment via a pathway involving the Ctf18-RFC complex and Mrc1p; stimulates Top3p DNA catenation/decatenation activity; null mutants display increased rates of recombination and delayed S phase (1, 3, 4, 5)
Name Description RecQ Mediated genome Instability 1, 2
Chromosomal Location
ChrXVI:503517 to 504242 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All RMI1 GO evidence and references
  View Computational GO annotations for RMI1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 7 genes
Resources
Classical genetics
conditional
null
Large-scale survey
null
Resources
182 total interaction(s) for 118 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 1
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 8
  • Biochemical Activity: 1
  • Co-fractionation: 3
  • Co-purification: 1
  • Reconstituted Complex: 2

Genetic Interactions
  • Dosage Lethality: 1
  • Negative Genetic: 82
  • Phenotypic Enhancement: 5
  • Phenotypic Suppression: 1
  • Positive Genetic: 8
  • Synthetic Growth Defect: 19
  • Synthetic Lethality: 38
  • Synthetic Rescue: 11

Resources
Expression Summary
histogram
Resources
Length (a.a.) 241
Molecular Weight (Da) 26,859
Isoelectric Point (pI) 9.73
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXVI:503517 to 504242 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..726 503517..504242 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005945
References cited on this page View Complete Literature Guide for RMI1
1) Chang M, et al.  (2005) RMI1/NCE4, a suppressor of genome instability, encodes a member of the RecQ helicase/Topo III complex. EMBO J 24(11):2024-33
2) Mullen JR, et al.  (2005) Yeast Rmi1/Nce4 controls genome stability as a subunit of the Sgs1-Top3 complex. Mol Cell Biol 25(11):4476-87
3) Chen CF and Brill SJ  (2007) Binding and activation of DNA topoisomerase III by the Rmi1 subunit. J Biol Chem 282(39):28971-9
4) Cejka P, et al.  (2012) Decatenation of DNA by the S. cerevisiae Sgs1-Top3-Rmi1 and RPA complex: a mechanism for disentangling chromosomes. Mol Cell 47(6):886-96
5) Lai MS, et al.  (2012) Rmi1 functions in S phase-mediated cohesion establishment via a pathway involving the Ctf18-RFC complex and Mrc1. Biochem Biophys Res Commun 427(3):682-6