Standard Name	RMI1 1, 2
Systematic Name	YPL024W
Alias	NCE4
Feature Type	ORF, Verified
Description	Subunit of the RecQ (Sgs1p) - Topo III (Top3p) complex; stimulates superhelical relaxing and ssDNA binding activities of Top3p; involved in response to DNA damage; functions in S phase-mediated cohesion establishment via a pathway involving the Ctf18-RFC complex and Mrc1p; null mutants display increased rates of recombination and delayed S phase (1, 3, 4)
Name Description	RecQ Mediated genome Instability 1, 2

Chromosomal Location	ChrXVI:503517 to 504242 | ORF Map | GBrowse

	View Computational GO annotations for RMI1
Molecular Function
Manually curated	enzyme activator activity (IDA) four-way junction DNA binding (IDA) single-stranded DNA binding (IDA)
Biological Process
Manually curated	mitotic sister chromatid cohesion (IGI, IMP) positive regulation of DNA binding (IDA) response to DNA damage stimulus (IGI)
Cellular Component
Manually curated	RecQ helicase-Topo III complex (IDA, IPI)
High-throughput	cytoplasm (IDA) nucleus (IDA)

Classical genetics
conditional	cell cycle progression through the G2/M phase transition: delayed heat sensitivity: increased resistance to methyl methanesulfonate: decreased resistance to hydroxyurea: decreased
null	cell cycle progression through the G2/M phase transition: delayed cellular morphology: abnormal chromosome/plasmid maintenance: abnormal colony sectoring: increased mutation frequency: increased resistance to benomyl: decreased vegetative growth: decreased
Large-scale survey
null	colony sectoring: increased competitive fitness: decreased filamentous growth: decreased invasive growth: absent mitotic recombination: increased Rad52-YFP distribution: increased resistance to bleomycin: increased resistance to fluconazole: increased resistance to hydroxyurea: decreased resistance to 5-fluorouracil: decreased resistance to methyl methanesulfonate: decreased resistance to camptothecin: decreased resistance to cycloheximide: decreased resistance to caffeine: decreased resistance to mycophenolic acid: decreased resistance to tirapazamine: decreased resistance to doxorubicin: decreased resistance to sorbate: decreased resistance to paromomycin: decreased respiratory growth: decreased rate transposable element transposition: increased vegetative growth: decreased rate viable
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	181 total interaction(s) for 117 unique genes/features.
Physical Interactions	Affinity Capture-MS: 1 Affinity Capture-RNA: 1 Affinity Capture-Western: 8 Biochemical Activity: 1 Co-fractionation: 3 Co-purification: 1 Reconstituted Complex: 2
Genetic Interactions	Dosage Lethality: 1 Negative Genetic: 82 Phenotypic Enhancement: 5 Phenotypic Suppression: 1 Positive Genetic: 8 Synthetic Growth Defect: 18 Synthetic Lethality: 38 Synthetic Rescue: 11
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP
Homologs	Ashbya (AGD) | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrXVI:503517 to 504242 | ORF Map | GBrowse
Last Update	Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..726 503517..504242 2011-02-03 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI1631 S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain CLIB215 S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FL100 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain M22 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain T73 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain Y10 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000005945