RLI1/YDR091C Summary Help

Standard Name RLI1 1
Systematic Name YDR091C
Feature Type ORF, Verified
Description Essential Fe-S protein; required for ribosome biogenesis, translation initiation/termination; facilitates binding of multifactor complex (MFC) of initiation factors to small ribosomal subunit; Dom34-Hbs1 complex and Rli1p work in dissociating inactive ribosomes, thereby facilitating translation restart; forms complex with Lto1p and Yae1p; dependency on ROS-labile FeS clusters, activity in nuclear ribosomal-subunit export impaired by mild oxidative stress (2, 3, 4, 5, 6, 7)
Name Description RNase L Inhibitor
Chromosomal Location
ChrIV:628534 to 626708 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All RLI1 GO evidence and references
  View Computational GO annotations for RLI1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 7 genes
Resources
Classical genetics
conditional
null
overexpression
reduction of function
Large-scale survey
conditional
null
overexpression
reduction of function
Resources
154 total interaction(s) for 116 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 67
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 12
  • Biochemical Activity: 2
  • PCA: 3
  • Two-hybrid: 3

Genetic Interactions
  • Dosage Rescue: 1
  • Negative Genetic: 27
  • Phenotypic Enhancement: 1
  • Positive Genetic: 33
  • Synthetic Growth Defect: 2
  • Synthetic Lethality: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 608
Molecular Weight (Da) 68,340
Isoelectric Point (pI) 8.82
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIV:628534 to 626708 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1827 628534..626708 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002498
References cited on this page View Complete Literature Guide for RLI1
1) Rutkowski, L.H. and R.E. Esposito  (1999) personal communication
2) Dong J, et al.  (2004) The essential ATP-binding cassette protein RLI1 functions in translation by promoting preinitiation complex assembly. J Biol Chem 279(40):42157-68
3) Kispal G, et al.  (2005) Biogenesis of cytosolic ribosomes requires the essential iron-sulphur protein Rli1p and mitochondria. EMBO J 24(3):589-98
4) Khoshnevis S, et al.  (2010) The iron-sulphur protein RNase L inhibitor functions in translation termination. EMBO Rep 11(3):214-9
5) Alhebshi A, et al.  (2012) The essential iron-sulfur protein Rli1 is an important target accounting for inhibition of cell growth by reactive oxygen species. Mol Biol Cell 23(18):3582-90
6) Zhai C, et al.  (2014) The function of ORAOV1/LTO1, a gene that is overexpressed frequently in cancer: essential roles in the function and biogenesis of the ribosome. Oncogene 33(4):484-94
7) van den Elzen AM, et al.  (2014) Dom34-Hbs1 mediated dissociation of inactive 80S ribosomes promotes restart of translation after stress. EMBO J 33(3):265-76