RLF2/YPR018W Summary Help

Standard Name RLF2 1
Systematic Name YPR018W
Alias CAC1 2
Feature Type ORF, Verified
Description Largest subunit (p90) of the Chromatin Assembly Complex (CAF-1); chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; and chromatin dynamics during transcription (2, 3, 4, 5, 6, 7, 8, 9, 10, 11)
Name Description Rap1 protein Localization Factor 1
Chromosomal Location
ChrXVI:594476 to 596296 | ORF Map | GBrowse
Gene Ontology Annotations All RLF2 GO evidence and references
  View Computational GO annotations for RLF2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Targets 845 genes
Regulators 59 genes
Classical genetics
reduction of function
Large-scale survey
337 total interaction(s) for 186 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 12
  • Affinity Capture-Western: 16
  • Biochemical Activity: 1
  • PCA: 2
  • Reconstituted Complex: 6
  • Two-hybrid: 49

Genetic Interactions
  • Dosage Growth Defect: 2
  • Dosage Lethality: 1
  • Dosage Rescue: 3
  • Negative Genetic: 118
  • Phenotypic Enhancement: 34
  • Phenotypic Suppression: 2
  • Positive Genetic: 18
  • Synthetic Growth Defect: 54
  • Synthetic Lethality: 9
  • Synthetic Rescue: 10

Expression Summary
Length (a.a.) 606
Molecular Weight (Da) 70,209
Isoelectric Point (pI) 6.62
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXVI:594476 to 596296 | ORF Map | GBrowse
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1821 594476..596296 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000006222
References cited on this page View Complete Literature Guide for RLF2
1) Enomoto S, et al.  (1997) RLF2, a subunit of yeast chromatin assembly factor-I, is required for telomeric chromatin function in vivo. Genes Dev 11(3):358-70
2) Kaufman PD, et al.  (1997) Ultraviolet radiation sensitivity and reduction of telomeric silencing in Saccharomyces cerevisiae cells lacking chromatin assembly factor-I. Genes Dev 11(3):345-57
3) Enomoto S and Berman J  (1998) Chromatin assembly factor I contributes to the maintenance, but not the re-establishment, of silencing at the yeast silent mating loci. Genes Dev 12(2):219-32
4) Game JC and Kaufman PD  (1999) Role of Saccharomyces cerevisiae chromatin assembly factor-I in repair of ultraviolet radiation damage in vivo. Genetics 151(2):485-97
5) Smith JS, et al.  (1999) A genetic screen for ribosomal DNA silencing defects identifies multiple DNA replication and chromatin-modulating factors. Mol Cell Biol 19(4):3184-97
6) Sharp JA, et al.  (2002) Chromatin assembly factor I and Hir proteins contribute to building functional kinetochores in S. cerevisiae. Genes Dev 16(1):85-100
7) Glowczewski L, et al.  (2004) Yeast chromatin assembly complex 1 protein excludes nonacetylatable forms of histone H4 from chromatin and the nucleus. Mol Cell Biol 24(23):10180-92
8) Zabaronick SR and Tyler JK  (2005) The histone chaperone anti-silencing function 1 is a global regulator of transcription independent of passage through S phase. Mol Cell Biol 25(2):652-60
9) Zhou H, et al.  (2006) Chromatin assembly factor 1 interacts with histone H3 methylated at lysine 79 in the processes of epigenetic silencing and DNA repair. Biochemistry 45(9):2852-61
10) Kim JA and Haber JE  (2009) Chromatin assembly factors Asf1 and CAF-1 have overlapping roles in deactivating the DNA damage checkpoint when DNA repair is complete. Proc Natl Acad Sci U S A 106(4):1151-6
11) Kim HJ, et al.  (2009) Potential role of the histone chaperone, CAF-1, in transcription. BMB Rep 42(4):227-31