RLF2/YPR018W Summary

RLF2/YPR018W Summary

Standard Name	RLF2 1
Systematic Name	YPR018W
Alias	CAC1 2
Feature Type	ORF, Verified
Description	Largest subunit (p90) of the Chromatin Assembly Complex (CAF-1); chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; and chromatin dynamics during transcription (2, 3, 4, 5, 6, 7, 8, 9, 10, 11)
Name Description	Rap1 protein Localization Factor 1

Chromosomal Location	ChrXVI:594476 to 596296 \| ORF Map \| GBrowse

Gene Ontology Annotations All RLF2 GO evidence and references

	View Computational GO annotations for RLF2
Molecular Function
Manually curated	contributes_to histone binding (IDA)
Biological Process
Manually curated	DNA replication-dependent nucleosome assembly (IDA, IMP)
Cellular Component
Manually curated	CAF-1 complex (IDA) chromosome, centromeric region (IDA) nucleus (IDA)

Mutant phenotypes All RLF2 Phenotype evidence and references

Classical genetics
null	UV resistance: decreased chromosome/plasmid maintenance: abnormal replicative lifespan: decreased resistance to camptothecin: decreased silencing: abnormal viable
reduction of function	silencing: decreased
Large-scale survey
null	anaerobic growth: decreased competitive fitness: decreased heat sensitivity: increased resistance to mycophenolic acid: increased resistance to methyl methanesulfonate: increased resistance to fluconazole: increased resistance to cycloheximide: increased resistance to camptothecin: increased resistance to methyl methanesulfonate: decreased resistance to caffeine: increased stress resistance: increased vacuolar morphology: abnormal viable
overexpression	vegetative growth: decreased rate
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All RLF2 Interaction evidence and references

	338 total interaction(s) for 188 unique genes/features.
Physical Interactions	Affinity Capture-MS: 12 Affinity Capture-Western: 16 Biochemical Activity: 1 PCA: 2 Reconstituted Complex: 6 Two-hybrid: 49
Genetic Interactions	Dosage Growth Defect: 2 Dosage Lethality: 1 Dosage Rescue: 3 Negative Genetic: 118 Phenotypic Enhancement: 35 Phenotypic Suppression: 2 Positive Genetic: 19 Synthetic Growth Defect: 53 Synthetic Lethality: 9 Synthetic Rescue: 10
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder \| YeastRC Two-Hybrid

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All RLF2 Protein evidence and references

Localization	YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrXVI:594476 to 596296 | |

SGD ORF map

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..1821	594476..596296	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000006222

References cited on this page View Complete Literature Guide for RLF2

1)	Enomoto S, et al. (1997) RLF2, a subunit of yeast chromatin assembly factor-I, is required for telomeric chromatin function in vivo. Genes Dev 11(3):358-70
2)	Kaufman PD, et al. (1997) Ultraviolet radiation sensitivity and reduction of telomeric silencing in Saccharomyces cerevisiae cells lacking chromatin assembly factor-I. Genes Dev 11(3):345-57
3)	Enomoto S and Berman J (1998) Chromatin assembly factor I contributes to the maintenance, but not the re-establishment, of silencing at the yeast silent mating loci. Genes Dev 12(2):219-32
4)	Game JC and Kaufman PD (1999) Role of Saccharomyces cerevisiae chromatin assembly factor-I in repair of ultraviolet radiation damage in vivo. Genetics 151(2):485-97
5)	Smith JS, et al. (1999) A genetic screen for ribosomal DNA silencing defects identifies multiple DNA replication and chromatin-modulating factors. Mol Cell Biol 19(4):3184-97
6)	Sharp JA, et al. (2002) Chromatin assembly factor I and Hir proteins contribute to building functional kinetochores in S. cerevisiae. Genes Dev 16(1):85-100
7)	Glowczewski L, et al. (2004) Yeast chromatin assembly complex 1 protein excludes nonacetylatable forms of histone H4 from chromatin and the nucleus. Mol Cell Biol 24(23):10180-92
8)	Zabaronick SR and Tyler JK (2005) The histone chaperone anti-silencing function 1 is a global regulator of transcription independent of passage through S phase. Mol Cell Biol 25(2):652-60
9)	Zhou H, et al. (2006) Chromatin assembly factor 1 interacts with histone H3 methylated at lysine 79 in the processes of epigenetic silencing and DNA repair. Biochemistry 45(9):2852-61
10)	Kim JA and Haber JE (2009) Chromatin assembly factors Asf1 and CAF-1 have overlapping roles in deactivating the DNA damage checkpoint when DNA repair is complete. Proc Natl Acad Sci U S A 106(4):1151-6
11)	Kim HJ, et al. (2009) Potential role of the histone chaperone, CAF-1, in transcription. BMB Rep 42(4):227-31