RLF2/YPR018W Summary Help

Standard Name RLF2 1
Systematic Name YPR018W
Alias CAC1 2
Feature Type ORF, Verified
Description Largest subunit (p90) of the Chromatin Assembly Complex (CAF-1); chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; chromatin dynamics during transcription; and repression of divergent noncoding transcription (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
Name Description Rap1 protein Localization Factor 1
Chromosomal Location
ChrXVI:594476 to 596296 | ORF Map | GBrowse
Gene Ontology Annotations All RLF2 GO evidence and references
  View Computational GO annotations for RLF2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 5 genes
Classical genetics
reduction of function
Large-scale survey
337 total interaction(s) for 186 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 12
  • Affinity Capture-Western: 16
  • Biochemical Activity: 1
  • PCA: 2
  • Reconstituted Complex: 6
  • Two-hybrid: 49

Genetic Interactions
  • Dosage Growth Defect: 2
  • Dosage Lethality: 1
  • Dosage Rescue: 3
  • Negative Genetic: 118
  • Phenotypic Enhancement: 34
  • Phenotypic Suppression: 2
  • Positive Genetic: 18
  • Synthetic Growth Defect: 54
  • Synthetic Lethality: 9
  • Synthetic Rescue: 10

Expression Summary
Length (a.a.) 606
Molecular Weight (Da) 70,209
Isoelectric Point (pI) 6.62
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXVI:594476 to 596296 | ORF Map | GBrowse
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1821 594476..596296 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000006222
References cited on this page View Complete Literature Guide for RLF2
1) Enomoto S, et al.  (1997) RLF2, a subunit of yeast chromatin assembly factor-I, is required for telomeric chromatin function in vivo. Genes Dev 11(3):358-70
2) Kaufman PD, et al.  (1997) Ultraviolet radiation sensitivity and reduction of telomeric silencing in Saccharomyces cerevisiae cells lacking chromatin assembly factor-I. Genes Dev 11(3):345-57
3) Enomoto S and Berman J  (1998) Chromatin assembly factor I contributes to the maintenance, but not the re-establishment, of silencing at the yeast silent mating loci. Genes Dev 12(2):219-32
4) Game JC and Kaufman PD  (1999) Role of Saccharomyces cerevisiae chromatin assembly factor-I in repair of ultraviolet radiation damage in vivo. Genetics 151(2):485-97
5) Smith JS, et al.  (1999) A genetic screen for ribosomal DNA silencing defects identifies multiple DNA replication and chromatin-modulating factors. Mol Cell Biol 19(4):3184-97
6) Sharp JA, et al.  (2002) Chromatin assembly factor I and Hir proteins contribute to building functional kinetochores in S. cerevisiae. Genes Dev 16(1):85-100
7) Glowczewski L, et al.  (2004) Yeast chromatin assembly complex 1 protein excludes nonacetylatable forms of histone H4 from chromatin and the nucleus. Mol Cell Biol 24(23):10180-92
8) Zabaronick SR and Tyler JK  (2005) The histone chaperone anti-silencing function 1 is a global regulator of transcription independent of passage through S phase. Mol Cell Biol 25(2):652-60
9) Zhou H, et al.  (2006) Chromatin assembly factor 1 interacts with histone H3 methylated at lysine 79 in the processes of epigenetic silencing and DNA repair. Biochemistry 45(9):2852-61
10) Kim JA and Haber JE  (2009) Chromatin assembly factors Asf1 and CAF-1 have overlapping roles in deactivating the DNA damage checkpoint when DNA repair is complete. Proc Natl Acad Sci U S A 106(4):1151-6
11) Kim HJ, et al.  (2009) Potential role of the histone chaperone, CAF-1, in transcription. BMB Rep 42(4):227-31
12) Marquardt S, et al.  (2014) A chromatin-based mechanism for limiting divergent noncoding transcription. Cell 157(7):1712-23