RIA1/YNL163C Summary Help

Standard Name RIA1 1
Systematic Name YNL163C
Alias EFL1
Feature Type ORF, Verified
Description Cytoplasmic GTPase/eEF2-like factor involved in ribosomal biogenesis; with Sdo1p, a guanine nucleotide exchange factor (GEF), promotes release of Tif6p from 60S ribosomal subunits in the cytoplasm so that they can assemble with 40S subunits to generate mature ribosomes; required for quality control check of newly made large ribosomal subunits before they are released into the pool of translating ribosomes (1, 2, 3, 4, 5, 6, 7)
Name Description RIbosome Assembly 1
Chromosomal Location
ChrXIV:330073 to 326741 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All RIA1 GO evidence and references
  View Computational GO annotations for RIA1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 2 genes
Classical genetics
Large-scale survey
reduction of function
228 total interaction(s) for 219 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 3
  • Affinity Capture-RNA: 4
  • Co-fractionation: 2
  • Co-purification: 1
  • Two-hybrid: 1

Genetic Interactions
  • Dosage Rescue: 1
  • Negative Genetic: 134
  • Positive Genetic: 61
  • Synthetic Growth Defect: 17
  • Synthetic Rescue: 4

Expression Summary
Length (a.a.) 1,110
Molecular Weight (Da) 124,462
Isoelectric Point (pI) 4.86
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXIV:330073 to 326741 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..3333 330073..326741 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005107
References cited on this page View Complete Literature Guide for RIA1
1) Becam AM, et al.  (2001) Ria1p (Ynl163c), a protein similar to elongation factors 2, is involved in the biogenesis of the 60S subunit of the ribosome in Saccharomyces cerevisiae. Mol Genet Genomics 266(3):454-62
2) Senger B, et al.  (2001) The nucle(ol)ar Tif6p and Efl1p are required for a late cytoplasmic step of ribosome synthesis. Mol Cell 8(6):1363-73
3) Menne TF, et al.  (2007) The Shwachman-Bodian-Diamond syndrome protein mediates translational activation of ribosomes in yeast. Nat Genet 39(4):486-95
4) Gartmann M, et al.  (2010) Mechanism of eIF6-mediated inhibition of ribosomal subunit joining. J Biol Chem 285(20):14848-51
5) Finch AJ, et al.  (2011) Uncoupling of GTP hydrolysis from eIF6 release on the ribosome causes Shwachman-Diamond syndrome. Genes Dev 25(9):917-29
6) Bussiere C, et al.  (2012) Integrity of the P-site is probed during maturation of the 60S ribosomal subunit. J Cell Biol 197(6):747-59
7) Gijsbers A, et al.  (2013) Guanine nucleotide exchange in the ribosomal GTPase EFL1 is modulated by the protein mutated in the Shwachman-Diamond Syndrome. Biochem Biophys Res Commun 437(3):349-54