RHO4/YKR055W Summary Help

Standard Name RHO4 1
Systematic Name YKR055W
Feature Type ORF, Verified
Description Non-essential small GTPase; member of the Rho/Rac subfamily of Ras-like proteins; likely to be involved in the establishment of cell polarity; has long N-terminal extension that plays an important role in Rho4p function and is shared with Rho4 homologs in other yeasts and filamentous fungi (2, 3, 4 and see Summary Paragraph)
Name Description Ras HOmolog 1
Chromosomal Location
ChrXI:548216 to 549091 | ORF Map | GBrowse
Gene Ontology Annotations All RHO4 GO evidence and references
  View Computational GO annotations for RHO4
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 4 genes
Classical genetics
Large-scale survey
100 total interaction(s) for 69 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 3
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 1
  • Biochemical Activity: 2
  • Reconstituted Complex: 1
  • Two-hybrid: 8

Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Rescue: 9
  • Negative Genetic: 56
  • Phenotypic Enhancement: 2
  • Synthetic Growth Defect: 5
  • Synthetic Lethality: 5
  • Synthetic Rescue: 6

Expression Summary
Length (a.a.) 291
Molecular Weight (Da) 32,185
Isoelectric Point (pI) 6.66
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXI:548216 to 549091 | ORF Map | GBrowse
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..876 548216..549091 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001763

RHO4 encodes a non-essential small GTPase in the Rho/Rac subfamily of Ras-like GTPases (5). Other S. cerevisiae Rho GTPases are encoded by RHO1, RHO2, RHO3, RHO5, and CDC42 (3). Like other eukaryotic Rho-type GTPases, Rho4p is likely to be involved in the establishment of cell polarity (3). Rho4p regulates interaction between Bnr1p and Hof1p, two proteins implicated in cytoskeletal organization (6). Deletion of RHO4 causes no apparent defect, but null mutations in RHO3 and RHO4 are synthetically lethal (5, 3). The GTPase activity of Rho4p is positively regulated by the GTPase activating protein (GAP) Rgd1p (2).

Note: The sequence first published for RHO4 1 is missing the first 61 codons (7). Experiments using the truncated RHO4 construct may give different results from those using the full-length construct.

2000-06-16 MIDORI (updated with assistance from Daniel R. TerBush)

Last updated: 1999-11-24 Contact SGD

References cited on this page View Complete Literature Guide for RHO4
1) Matsui Y and Toh-e A  (1992) Isolation and characterization of two novel ras superfamily genes in Saccharomyces cerevisiae. Gene 114(1):43-9
2) Doignon F, et al.  (1999) The yeast Rgd1p is a GTPase activating protein of the Rho3 and rho4 proteins. FEBS Lett 459(3):458-62
3) Madden K and Snyder M  (1998) Cell polarity and morphogenesis in budding yeast. Annu Rev Microbiol 52():687-744
4) Gong T, et al.  (2013) Control of polarized growth by the Rho family GTPase Rho4 in budding yeast: requirement of the N-terminal extension of Rho4 and regulation by the Rho GTPase-activating protein Bem2. Eukaryot Cell 12(2):368-77
5) Matsui Y and Toh-E A  (1992) Yeast RHO3 and RHO4 ras superfamily genes are necessary for bud growth, and their defect is suppressed by a high dose of bud formation genes CDC42 and BEM1. Mol Cell Biol 12(12):5690-9
6) Kamei T, et al.  (1998) Interaction of Bnr1p with a novel Src homology 3 domain-containing Hof1p. Implication in cytokinesis in Saccharomyces cerevisiae. J Biol Chem 273(43):28341-5
7) Van Vliet-Reedijk JC and Planta RJ  (1993) The RHO4a and NUD1 genes on Saccharomyces cerevisiae chromosome XI. Yeast 9(10):1139-47