RHO4/YKR055W Summary Help

Standard Name RHO4
Systematic Name YKR055W
Feature Type ORF, Verified
Description Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins, likely to be involved in the establishment of cell polarity (1, 2 and see Summary Paragraph)
Name Description Ras HOmolog 3
Chromosomal Location
ChrXI:548216 to 549091 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All RHO4 GO evidence and references
  View Computational GO annotations for RHO4
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Mutant phenotypes All RHO4 Phenotype evidence and references
Classical genetics
null
Large-scale survey
null
Resources
interactions All RHO4 Interaction evidence and references
97 total interaction(s) for 67 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 1
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 1
  • Biochemical Activity: 2
  • Reconstituted Complex: 1
  • Two-hybrid: 8
Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Rescue: 9
  • Negative Genetic: 56
  • Phenotypic Enhancement: 2
  • Synthetic Growth Defect: 4
  • Synthetic Lethality: 5
  • Synthetic Rescue: 6
Resources
Expression Summary
histogram
Resources
Protein Information All RHO4 Protein evidence and references
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXI:548216 to 549091 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..876 548216..549091 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001763
SUMMARY PARAGRAPH for RHO4

RHO4 encodes a non-essential small GTPase in the Rho/Rac subfamily of Ras-like GTPases (4). Other S. cerevisiae Rho GTPases are encoded by RHO1, RHO2, RHO3, RHO5, and CDC42 (2). Like other eukaryotic Rho-type GTPases, Rho4p is likely to be involved in the establishment of cell polarity (2). Rho4p regulates interaction between Bnr1p and Hof1p, two proteins implicated in cytoskeletal organization (5). Deletion of RHO4 causes no apparent defect, but null mutations in RHO3 and RHO4 are synthetically lethal (4, 2). The GTPase activity of Rho4p is positively regulated by the GTPase activating protein (GAP) Rgd1p (1).

Note: The sequence first published for RHO4 3 is missing the first 61 codons (6). Experiments using the truncated RHO4 construct may give different results from those using the full-length construct.

2000-06-16 MIDORI (updated with assistance from Daniel R. TerBush)

Last updated: 1999-11-24

References cited on this page View Complete Literature Guide for RHO4
1) Doignon F, et al.  (1999) The yeast Rgd1p is a GTPase activating protein of the Rho3 and rho4 proteins. FEBS Lett 459(3):458-62
2) Madden K and Snyder M  (1998) Cell polarity and morphogenesis in budding yeast. Annu Rev Microbiol 52():687-744
3) Matsui Y and Toh-e A  (1992) Isolation and characterization of two novel ras superfamily genes in Saccharomyces cerevisiae. Gene 114(1):43-9
4) Matsui Y and Toh-E A  (1992) Yeast RHO3 and RHO4 ras superfamily genes are necessary for bud growth, and their defect is suppressed by a high dose of bud formation genes CDC42 and BEM1. Mol Cell Biol 12(12):5690-9
5) Kamei T, et al.  (1998) Interaction of Bnr1p with a novel Src homology 3 domain-containing Hof1p. Implication in cytokinesis in Saccharomyces cerevisiae. J Biol Chem 273(43):28341-5
6) Van Vliet-Reedijk JC and Planta RJ  (1993) The RHO4a and NUD1 genes on Saccharomyces cerevisiae chromosome XI. Yeast 9(10):1139-47