RHO3/YIL118W Summary Help

RHO3 BASIC INFORMATION

Standard Name RHO3
Systematic Name YIL118W
Feature Type ORF, Verified
Description Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins involved in the establishment of cell polarity; GTPase activity positively regulated by the GTPase activating protein (GAP) Rgd1p (1, 2 and see Summary Paragraph)
Name Description Ras HOmolog 3
GO Annotations All RHO3 GO evidence and references
    View Computational GO annotations for RHO3
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Mutant Phenotype All RHO3 Phenotype details and references
Classical genetics
conditional
Large-scale survey
null
Interactions RHO3 All interactions details and references
76 total interaction(s) for 41 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 4
  • Affinity Capture-RNA: 2
  • Biochemical Activity: 1
  • Reconstituted Complex: 7
  • Two-hybrid: 10
Genetic Interactions
  • Dosage Lethality: 1
  • Dosage Rescue: 38
  • Synthetic Growth Defect: 3
  • Synthetic Lethality: 8
  • Synthetic Rescue: 2
Sequence Information
ChrIX:139749 to 140444 | ORF Map | GBrowse
Gbrowse
Last Update Coordinates: 1994-12-10 | Sequence: 1994-12-10
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..696 139749..140444 1994-12-10 1994-12-10
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | UniProtKB
Primary SGDIDS000001380

RHO3 RESOURCES

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SGD ORF mapGBrowse
SGD ORF map
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  • Localization Resources
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  • Functional Analysis

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Expression Summary histogram

ADDITIONAL INFORMATION for RHO3

SUMMARY PARAGRAPH for RHO3

RHO3 encodes a non-essential small GTPase in the Rho/Rac subfamily of Ras-like GTPases (4). Other S. cerevisiae Rho GTPases are encoded by RHO1, RHO2, RHO4, RHO5, and CDC42 (2). Like other eukaryotic Rho-type GTPases, Rho3p is involved in the establishment of cell polarity (2). Temperature sensitive rho3 mutants lose cell polarity at the restrictive temperature; a dominant RHO3 allele causes cold sensitivity and aberrant cell morphology (5). RHO3 interacts genetically with SEC4, which encodes a Rab-type small GTPase, suggesting that Rho3p may regulate polarized secretion (5, 2). rho3 and rho4 null mutations are synthetically lethal (4, 2). The GTPase activity of Rho3p is positively regulated by the GTPase activating protein (GAP) Rgd1p (1).

Last updated: 1999-11-24

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for RHO3]

1) Doignon F, et al.  (1999) The yeast Rgd1p is a GTPase activating protein of the Rho3 and rho4 proteins. FEBS Lett 459(3):458-62
2) Madden K and Snyder M  (1998) Cell polarity and morphogenesis in budding yeast. Annu Rev Microbiol 52():687-744
3) Matsui Y and Toh-e A  (1992) Isolation and characterization of two novel ras superfamily genes in Saccharomyces cerevisiae. Gene 114(1):43-9
4) Matsui Y and Toh-E A  (1992) Yeast RHO3 and RHO4 ras superfamily genes are necessary for bud growth, and their defect is suppressed by a high dose of bud formation genes CDC42 and BEM1. Mol Cell Biol 12(12):5690-9
5) Imai J, et al.  (1996) Genetic analysis of the Saccharomyces cerevisiae RHO3 gene, encoding a rho-type small GTPase, provides evidence for a role in bud formation. Genetics 142(2):359-69