RHO1/YPR165W Summary 
| Standard Name | RHO1 1 |
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| Systematic Name | YPR165W |
| Feature Type | ORF, Verified |
| Description | GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p) (2, 3, 4 and see Summary Paragraph) |
| Name Description | Ras HOmolog 1 |
| Chromosomal Location | |
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| View Computational GO annotations for RHO1 | |
| Molecular Function | |
| Manually curated | |
| Biological Process | |
| Manually curated |
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| Cellular Component | |
| Manually curated | |
| High-throughput |
| Classical genetics | |
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| overexpression | |
| Large-scale survey | |
| null | |
| Resources |
| 144 total interaction(s) for 79 unique genes/features. | |
| Physical Interactions |
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| Genetic Interactions |
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| Resources |
| Localization | |
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| Phosphorylation | PhosphoGRID | PhosphoPep Database |
| Structure | |
| Homologs |
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| Last Update | Coordinates: 2011-02-03 | Sequence: 1996-07-31 | ||||||||||||
| Subfeature details |
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| S288C only | |
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| S288C vs. other species | |
| S288C vs. other strains |
| External Links | All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB |
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| Primary SGDID | S000006369 |
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RHO1 encodes an essential small GTPase in the Rho/Rac subfamily of Ras-like GTPases (5). Other S. cerevisiae Rho GTPases are encoded by RHO2, RHO3, RHO4, RHO5, and CDC42 (6). Like other eukaryotic Rho-type GTPases, Rho1p is involved in the establishment of cell polarity, playing a role in the reorganization of the actin cytoskeleton (6). Rho1p regulates protein kinase C (encoded by PKC1) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (encoded by FKS1 and GSC2) (3, 4). Rho1p is localized to the plasma membrane at sites of growth such as incipient bud sites, bud tips, and the bud neck during cytokinesis (5). The localization of Rho1p is aberrent in sla1 null mutant cells (7). At the restrictive temperature, some temperature-sensitive rho1 mutants arrest with small buds (5); other temperature-sensitive rho1 alleles cause arrest as unbudded cells (2). The GTPase activity of Rho1p is regulated by the GTPase activating proteins (GAPs) Bem2p, Sac7p, Bag2p, and Rdi1p, and the guanine nucleotide exchange factors (GEFs) Rom1p and Rom2p (6).
Last updated: 1999-11-24 
| 1) | Madaule P, et al. (1987) Characterization of two members of the rho gene family from the yeast Saccharomyces cerevisiae. Proc Natl Acad Sci U S A 84(3):779-83 |
| 2) | Drgonova J, et al. (1999) The GTP-binding protein Rho1p is required for cell cycle progression and polarization of the yeast cell. J Cell Biol 146(2):373-87 |
| 3) | Drgonova J, et al. (1996) Rho1p, a yeast protein at the interface between cell polarization and morphogenesis. Science 272(5259):277-9 |
| 4) | Qadota H, et al. (1996) Identification of yeast Rho1p GTPase as a regulatory subunit of 1,3-beta-glucan synthase. Science 272(5259):279-81 |
| 5) | Yamochi W, et al. (1994) Growth site localization of Rho1 small GTP-binding protein and its involvement in bud formation in Saccharomyces cerevisiae. J Cell Biol 125(5):1077-93 |
| 6) | Madden K and Snyder M (1998) Cell polarity and morphogenesis in budding yeast. Annu Rev Microbiol 52():687-744 |
| 7) | Ayscough KR, et al. (1999) Sla1p is a functionally modular component of the yeast cortical actin cytoskeleton required for correct localization of both Rho1p-GTPase and Sla2p, a protein with talin homology. Mol Biol Cell 10(4):1061-75 |
