RGA1/YOR127W Summary Help

Standard Name RGA1 1
Systematic Name YOR127W
Alias DBM1 2 , THE1
Feature Type ORF, Verified
Description GTPase-activating protein for polarity-establishment protein Cdc42p; implicated in control of septin organization, pheromone response, and haploid invasive growth; relocalizes from bud neck to cytoplasm upon DNA replication stress; RGA1 has a paralog, RGA2, that arose from the whole genome duplication (1, 3, 4, 5)
Name Description Rho GTPase Activating Protein 1
Chromosomal Location
ChrXV:561170 to 564193 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All RGA1 GO evidence and references
  View Computational GO annotations for RGA1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 2 genes
Resources
Classical genetics
null
Large-scale survey
null
overexpression
Resources
126 total interaction(s) for 77 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 8
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 2
  • Biochemical Activity: 2
  • PCA: 1
  • Reconstituted Complex: 1
  • Two-hybrid: 24

Genetic Interactions
  • Dosage Rescue: 4
  • Negative Genetic: 61
  • Phenotypic Enhancement: 4
  • Phenotypic Suppression: 3
  • Positive Genetic: 3
  • Synthetic Growth Defect: 5
  • Synthetic Lethality: 4
  • Synthetic Rescue: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 1,007
Molecular Weight (Da) 112,830
Isoelectric Point (pI) 6.36
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXV:561170 to 564193 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..3024 561170..564193 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005653
References cited on this page View Complete Literature Guide for RGA1
1) Stevenson BJ, et al.  (1995) Mutation of RGA1, which encodes a putative GTPase-activating protein for the polarity-establishment protein Cdc42p, activates the pheromone-response pathway in the yeast Saccharomyces cerevisiae. Genes Dev 9(23):2949-63
2) Chen GC, et al.  (1996) The LIM domain-containing Dbm1 GTPase-activating protein is required for normal cellular morphogenesis in Saccharomyces cerevisiae. Mol Cell Biol 16(4):1376-90
3) Smith GR, et al.  (2002) GTPase-activating proteins for Cdc42. Eukaryot Cell 1(3):469-80
4) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
5) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76