RFX1/YLR176C Summary Help

Standard Name RFX1
Systematic Name YLR176C
Alias CRT1 1
Feature Type ORF, Verified
Description Major transcriptional repressor of DNA-damage-regulated genes; recruits repressors Tup1p and Cyc8p to their promoters; involved in DNA damage and replication checkpoint pathway; similar to a family of mammalian DNA binding RFX1-4 proteins (1, 2, 3)
Chromosomal Location
ChrXII:510232 to 507797 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All RFX1 GO evidence and references
  View Computational GO annotations for RFX1
Molecular Function
Manually curated
High-throughput
Biological Process
Manually curated
Cellular Component
High-throughput
Targets 597 genes
Regulators 3 genes
Resources
Classical genetics
null
Large-scale survey
null
overexpression
Resources
127 total interaction(s) for 98 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 14
  • Affinity Capture-RNA: 1
  • Reconstituted Complex: 7

Genetic Interactions
  • Dosage Growth Defect: 2
  • Dosage Lethality: 6
  • Dosage Rescue: 1
  • Negative Genetic: 55
  • Phenotypic Enhancement: 12
  • Phenotypic Suppression: 10
  • Positive Genetic: 3
  • Synthetic Growth Defect: 6
  • Synthetic Lethality: 1
  • Synthetic Rescue: 9

Resources
Expression Summary
histogram
Resources
Length (a.a.) 811
Molecular Weight (Da) 90,583
Isoelectric Point (pI) 9.18
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXII:510232 to 507797 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2436 510232..507797 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004166
References cited on this page View Complete Literature Guide for RFX1
1) Huang M, et al.  (1998) The DNA replication and damage checkpoint pathways induce transcription by inhibition of the Crt1 repressor. Cell 94(5):595-605
2) Emery P, et al.  (1996) RFX proteins, a novel family of DNA binding proteins conserved in the eukaryotic kingdom. Nucleic Acids Res 24(5):803-7
3) Woolstencroft RN, et al.  (2006) Ccr4 contributes to tolerance of replication stress through control of CRT1 mRNA poly(A) tail length. J Cell Sci 119(Pt 24):5178-92
4) Badis G, et al.  (2008) A library of yeast transcription factor motifs reveals a widespread function for Rsc3 in targeting nucleosome exclusion at promoters. Mol Cell 32(6):878-87
5) Zaim J, et al.  (2005) Identification of new genes regulated by the Crt1 transcription factor, an effector of the DNA damage checkpoint pathway in Saccharomyces cerevisiae. J Biol Chem 280(1):28-37
6) Harbison CT, et al.  (2004) Transcriptional regulatory code of a eukaryotic genome. Nature 431(7004):99-104