RFU1/YLR073C Summary Help

Standard Name RFU1 1
Systematic Name YLR073C
Feature Type ORF, Verified
Description Protein that inhibits Doa4p deubiquitinating activity; contributes to ubiquitin homeostasis by regulating the conversion of free ubiquitin chains to ubiquitin monomers by Doa4p; GFP-fusion protein localizes to endosomes (1, 2)
Name Description Regulator of Free Ubiquitin chains 1
Chromosomal Location
ChrXII:281621 to 281019 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All RFU1 GO evidence and references
  View Computational GO annotations for RFU1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 3 genes
Resources
Classical genetics
overexpression
Large-scale survey
null
overexpression
Resources
33 total interaction(s) for 20 unique genes/features.
Physical Interactions
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 3
  • Protein-RNA: 1
  • Reconstituted Complex: 2

Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Rescue: 1
  • Negative Genetic: 11
  • Phenotypic Enhancement: 3
  • Positive Genetic: 4
  • Synthetic Growth Defect: 3
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 200
Molecular Weight (Da) 22,850
Isoelectric Point (pI) 10
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXII:281621 to 281019 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..603 281621..281019 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004063
References cited on this page View Complete Literature Guide for RFU1
1) Kimura Y, et al.  (2009) An inhibitor of a deubiquitinating enzyme regulates ubiquitin homeostasis. Cell 137(3):549-59
2) Huh WK, et al.  (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91