RFC5/YBR087W Summary Help

Standard Name RFC5 1
Systematic Name YBR087W
Feature Type ORF, Verified
Description Subunit of heteropentameric Replication factor C (RF-C); RF-C is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon (2, 3)
Name Description Replication Factor C 2
Chromosomal Location
ChrII:423765 to 424829 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All RFC5 GO evidence and references
  View Computational GO annotations for RFC5
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 5 genes
Resources
Classical genetics
conditional
repressible
Large-scale survey
conditional
null
reduction of function
repressible
Resources
236 total interaction(s) for 148 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 46
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 14
  • Co-fractionation: 1
  • Co-purification: 6
  • FRET: 1
  • Reconstituted Complex: 6
  • Two-hybrid: 3

Genetic Interactions
  • Dosage Lethality: 1
  • Dosage Rescue: 9
  • Negative Genetic: 93
  • Phenotypic Enhancement: 8
  • Phenotypic Suppression: 4
  • Positive Genetic: 9
  • Synthetic Growth Defect: 16
  • Synthetic Lethality: 15
  • Synthetic Rescue: 3

Resources
Expression Summary
histogram
Resources
Length (a.a.) 354
Molecular Weight (Da) 39,941
Isoelectric Point (pI) 8.16
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrII:423765 to 424829 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1997-01-28
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1065 423765..424829 2011-02-03 1997-01-28
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000291
References cited on this page View Complete Literature Guide for RFC5
1) Gary SL and Burgers MJ  (1995) Identification of the fifth subunit of Saccharomyces cerevisiae replication factor C. Nucleic Acids Res 23(24):4986-91
2) Cullmann G, et al.  (1995) Characterization of the five replication factor C genes of Saccharomyces cerevisiae. Mol Cell Biol 15(9):4661-71
3) Yao N, et al.  (2003) Replication factor C clamp loader subunit arrangement within the circular pentamer and its attachment points to proliferating cell nuclear antigen. J Biol Chem 278(50):50744-53