RFC4/YOL094C Summary

RFC4 BASIC INFORMATION

Standard Name	RFC4
Systematic Name	YOL094C
Feature Type	ORF, Verified
Description	Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon (1, 2)
Name Description	Replication Factor C 1

GO Annotations	All RFC4 GO evidence and references
	View Computational GO annotations for RFC4
Molecular Function
Manually curated	contributes_to DNA clamp loader activity (IDA) purine nucleotide binding (ISS)
Biological Process
Manually curated	leading strand elongation (IDA) mismatch repair (TAS) sister chromatid cohesion (IPI)
Cellular Component
Manually curated	Ctf18 RFC-like complex (IPI) DNA replication factor C complex (IDA, ISS) Elg1 RFC-like complex (IPI) Rad17 RFC-like complex (IDA)

Mutant Phenotype	All RFC4 Phenotype details and references
Classical genetics
conditional	UV resistance: decreased chromosome segregation: abnormal cold sensitivity: increased resistance to hydroxyurea: decreased resistance to methyl methanesulfonate: decreased
unspecified	cell cycle progression in S phase: abnormal Rad53 accumulation: decreased resistance to methyl methanesulfonate: decreased resistance to hydroxyurea: decreased
Large-scale survey
null	inviable
repressible	cell cycle progression in G2 phase: abnormal

Interactions	RFC4 All interactions details and references
	127 total interaction(s) for 87 unique genes/features.
Physical Interactions	Affinity Capture-MS: 38 Affinity Capture-Western: 13 Co-fractionation: 1 Co-purification: 6 Reconstituted Complex: 4 Two-hybrid: 1
Genetic Interactions	Dosage Rescue: 1 Phenotypic Enhancement: 61 Phenotypic Suppression: 1 Synthetic Lethality: 1

Sequence Information

ChrXV:142555 to 141584 | |

Note: this feature is encoded on the Crick strand.

Last Update

Coordinates: 2006-01-05 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..972	142555..141584	2006-01-05	1996-07-31

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| UniProtKB

Primary SGDID	S000005454

RFC4 RESOURCES

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SGD ORF map	GBrowse

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Expression Summary

ADDITIONAL INFORMATION for RFC4

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for RFC4]

1)	Cullmann G, et al. (1995) Characterization of the five replication factor C genes of Saccharomyces cerevisiae. Mol Cell Biol 15(9):4661-71
2)	Yao N, et al. (2003) Replication factor C clamp loader subunit arrangement within the circular pentamer and its attachment points to proliferating cell nuclear antigen. J Biol Chem 278(50):50744-53

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