RFC2/YJR068W Summary Help

Standard Name RFC2 1
Systematic Name YJR068W
Feature Type ORF, Verified
Description Subunit of heteropentameric Replication factor C (RF-C); RF-C is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon (2, 3)
Name Description Replication Factor C 2
Chromosomal Location
ChrX:567643 to 568704 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All RFC2 GO evidence and references
  View Computational GO annotations for RFC2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 2 genes
Resources
Classical genetics
conditional
overexpression
reduction of function
repressible
Large-scale survey
conditional
null
overexpression
reduction of function
repressible
unspecified
Resources
103 total interaction(s) for 46 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 43
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 14
  • Co-purification: 18
  • FRET: 1
  • PCA: 2
  • Reconstituted Complex: 7
  • Two-hybrid: 4

Genetic Interactions
  • Dosage Rescue: 1
  • Negative Genetic: 2
  • Synthetic Growth Defect: 2
  • Synthetic Lethality: 6
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 353
Molecular Weight (Da) 39,741
Isoelectric Point (pI) 8.59
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrX:567643 to 568704 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1062 567643..568704 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003829
References cited on this page View Complete Literature Guide for RFC2
1) Noskov V, et al.  (1994) The RFC2 gene encoding a subunit of replication factor C of Saccharomyces cerevisiae. Nucleic Acids Res 22(9):1527-35
2) Cullmann G, et al.  (1995) Characterization of the five replication factor C genes of Saccharomyces cerevisiae. Mol Cell Biol 15(9):4661-71
3) Yao N, et al.  (2003) Replication factor C clamp loader subunit arrangement within the circular pentamer and its attachment points to proliferating cell nuclear antigen. J Biol Chem 278(50):50744-53