REV1/YOR346W Summary Help

Standard Name REV1 1, 2
Systematic Name YOR346W
Feature Type ORF, Verified
Description Deoxycytidyl transferase; involved in repair of abasic sites and adducted guanines in damaged DNA by translesion synthesis (TLS); forms a complex with the subunits of DNA polymerase zeta, Rev3p and Rev7p; relocalizes from nucleus to cytoplasm upon DNA replication stress (3, 4, 5, 6 and see Summary Paragraph)
Name Description REVersionless 1
Chromosomal Location
ChrXV:981828 to 984785 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All REV1 GO evidence and references
  View Computational GO annotations for REV1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 1 genes
Resources
Classical genetics
null
reduction of function
Large-scale survey
null
Resources
110 total interaction(s) for 55 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 1
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 13
  • Co-crystal Structure: 1
  • Co-purification: 1
  • Reconstituted Complex: 8
  • Two-hybrid: 9

Genetic Interactions
  • Negative Genetic: 23
  • Phenotypic Enhancement: 20
  • Phenotypic Suppression: 8
  • Positive Genetic: 2
  • Synthetic Growth Defect: 13
  • Synthetic Lethality: 8
  • Synthetic Rescue: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 985
Molecular Weight (Da) 112,226
Isoelectric Point (pI) 8.49
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXV:981828 to 984785 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2958 981828..984785 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005873
SUMMARY PARAGRAPH for REV1

REV1 is involved in translesion synthesis during post-replication repair and double-strand break repair (7, 8). REV1 encodes a deoxycytidyl transferase, a DNA polymerase that preferentially incorporates a C across abasic sites or damaged bases (3, 8, 9). However, this catalytic activity may not be its only function in translesion synthesis (10, 5, 11, 12). In addition to its role in translesion synthesis, Rev1p has a non-catalytic role during double-strand break repair (13).

Like other members of the Y-family of DNA polymerases, Rev1p has low processivity and low fidelity (14). Rev1p interacts with polymerase zeta, encoded by REV3 and REV7, for efficient bypass and extension past the DNA lesion (3, 12, 15). This process can be error-free or error-prone depending on the damaged nucleotide and the inserted nucleotide (reviewed in 8). However, REV1 is primarily considered to be in an error-prone translesion pathway due to a decrease in mutation frequency in its absence (1, 16).

REV1's role may not be limited to its polymerase activity. Rev1p contains a BRCT domain and a carboxy-terminus region that is essential for its interaction with the subunits of pol zeta (8). A significant decrease in mutation frequencies is observed when these regions are mutated but not when its deoxycytidyl transferase activity is mutated (11, 17, 10, 18, 12). REV1 is also implicated in double-strand break repair (19. Mutations in the deoxycytidyl transferase activity do not affect the localization of the Rev1p-polzeta complex to double-strand break sites (13).

Several different mechanisms regulate Rev1p activity. Rev1p interacts with and is stimulated by PCNA, which is monoubiquitinated by Rad6p-Rad18p in response to DNA damage (20, 21, 22). Rev1p is cell cycle-regulated and phosphorylated by the Mec1p-Ddc2p kinase in response to various types of DNA damages (23, 24, 25).

Last updated: 2010-02-02 Contact SGD

References cited on this page View Complete Literature Guide for REV1
1) Lemontt JF  (1971) Mutants of yeast defective in mutation induced by ultraviolet light. Genetics 68(1):21-33
2) Larimer, F.  (1989) Personal Communication, Mortimer Map Edition 10
3) Nelson JR, et al.  (1996) Deoxycytidyl transferase activity of yeast REV1 protein. Nature 382(6593):729-31
4) Larimer FW, et al.  (1989) The REV1 gene of Saccharomyces cerevisiae: isolation, sequence, and functional analysis. J Bacteriol 171(1):230-7
5) Haracska L, et al.  (2001) Roles of yeast DNA polymerases delta and zeta and of Rev1 in the bypass of abasic sites. Genes Dev 15(8):945-54
6) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
7) Lawrence CW  (2004) Cellular functions of DNA polymerase zeta and Rev1 protein. Adv Protein Chem 69:167-203
8) Prakash S, et al.  (2005) Eukaryotic translesion synthesis DNA polymerases: specificity of structure and function. Annu Rev Biochem 74:317-53
9) Howell CA, et al.  (2007) Pre-steady-state kinetic studies of protein-template-directed nucleotide incorporation by the yeast rev1 protein. Biochemistry 46(46):13451-9
10) Nelson JR, et al.  (2000) Evidence for a second function for Saccharomyces cerevisiae Rev1p. Mol Microbiol 37(3):549-54
11) Otsuka C, et al.  (2005) Roles of the polymerase and BRCT domains of Rev1 protein in translesion DNA synthesis in yeast in vivo. Mutat Res 578(1-2):79-87
12) Acharya N, et al.  (2006) Complex formation with Rev1 enhances the proficiency of Saccharomyces cerevisiae DNA polymerase zeta for mismatch extension and for extension opposite from DNA lesions. Mol Cell Biol 26(24):9555-63
13) Hirano Y and Sugimoto K  (2006) ATR homolog Mec1 controls association of DNA polymerase zeta-Rev1 complex with regions near a double-strand break. Curr Biol 16(6):586-90
14) Haracska L, et al.  (2002) Yeast Rev1 protein is a G template-specific DNA polymerase. J Biol Chem 277(18):15546-51
15) Bao G and Kow YW  (2009) Effect of sequence context and direction of replication on AP site bypass in Saccharomyces cerevisiae. Mutat Res 669(1-2):147-54
16) Johnson RE, et al.  (1998) Identification of APN2, the Saccharomyces cerevisiae homolog of the major human AP endonuclease HAP1, and its role in the repair of abasic sites. Genes Dev 12(19):3137-43
17) Acharya N, et al.  (2005) Complex formation of yeast Rev1 and Rev7 proteins: a novel role for the polymerase-associated domain. Mol Cell Biol 25(21):9734-40
18) D'Souza S and Walker GC  (2006) Novel Role for the C Terminus of Saccharomyces cerevisiae Rev1 in Mediating Protein-Protein Interactions. Mol Cell Biol 26(21):8173-82
19) Kolas NK and Durocher D  (2006) DNA repair: DNA polymerase zeta and Rev1 break in. Curr Biol 16(8):R296-9
20) Ulrich HD  (2009) Regulating post-translational modifications of the eukaryotic replication clamp PCNA. DNA Repair (Amst) 8(4):461-9
21) Garg P and Burgers PM  (2005) Ubiquitinated proliferating cell nuclear antigen activates translesion DNA polymerases eta and REV1. Proc Natl Acad Sci U S A 102(51):18361-6
22) Wood A, et al.  (2007) A ubiquitin-binding motif in the translesion DNA polymerase Rev1 mediates its essential functional interaction with ubiquitinated proliferating cell nuclear antigen in response to DNA damage. J Biol Chem 282(28):20256-63
23) Waters LS and Walker GC  (2006) The critical mutagenic translesion DNA polymerase Rev1 is highly expressed during G(2)/M phase rather than S phase. Proc Natl Acad Sci U S A 103(24):8971-6
24) Sabbioneda S, et al.  (2007) Yeast Rev1 is cell cycle regulated, phosphorylated in response to DNA damage and its binding to chromosomes is dependent upon MEC1. DNA Repair (Amst) 6(1):121-7
25) Pages V, et al.  (2009) Role of DNA damage-induced replication checkpoint in promoting lesion bypass by translesion synthesis in yeast. Genes Dev 23(12):1438-49