REG2/YBR050C Summary Help

Standard Name REG2 1
Systematic Name YBR050C
Feature Type ORF, Verified
Description Regulatory subunit of the Glc7p type-1 protein phosphatase; involved with Reg1p, Glc7p, and Snf1p in regulation of glucose-repressible genes, also involved in glucose-induced proteolysis of maltose permease; REG2 has a paralog, REG1, that arose from the whole genome duplication (1, 2, 3 and see Summary Paragraph)
Name Description REsistance to Glucose repression 1
Chromosomal Location
ChrII:338199 to 337183 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All REG2 GO evidence and references
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 5 genes
Classical genetics
Large-scale survey
19 total interaction(s) for 14 unique genes/features.
Physical Interactions
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 2
  • Reconstituted Complex: 1
  • Two-hybrid: 3

Genetic Interactions
  • Dosage Growth Defect: 3
  • Dosage Lethality: 2
  • Dosage Rescue: 2
  • Negative Genetic: 1
  • Synthetic Growth Defect: 3
  • Synthetic Rescue: 1

Expression Summary
Length (a.a.) 338
Molecular Weight (Da) 38,747
Isoelectric Point (pI) 8.14
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrII:338199 to 337183 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2011-02-03 | Sequence: 1997-01-28
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1017 338199..337183 2011-02-03 1997-01-28
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000254

REG2 encodes a regulatory subunit of the Glc7p type-1 protein phosphatase (PP1) (2). Reg2p, and the similar protein Reg1p, are each involved in targeting Glc7p to substrates that are phosphorylated by the Snf1p kinase (1). Glc7p-Reg2p and Glc7p-Reg1p are also involved in the glucose-induced proteolysis of maltose permease (Mal11p, Mal21p, Mal31p, Mal41p and Mal61p) (2).

Glucose repression of REG2 is partially dependent on Mig1p and Mig2p (4), and induction of REG2 expression during the diauxic shift is dependent on Cat8p (5). REG2 is not essential for viability (1), but reg2 null mutants display reduced rates of both glucose-induced proteolysis of maltose permease and inactivation of maltose transport (2), as well as reduced expression of PIS1 and INO1 during growth on glucose (6). reg1 reg2 double null mutants exhibit a severe growth defect as compared to either wild type or reg1 null mutants (1).

Overexpression of REG2 complements the slow-growth defect of a reg1 mutant (1), but does not complement defects in glycogen accumulation or glucose repression displayed by reg1 nulls (1). Overexpression of REG2 in a reg1 null mutant does restore the glucose-induced proteolysis of maltose permease and partially reinstates the inactivated maltose transport, but does not affect the insensitivity of MAL gene expression to repression by glucose (2).

Last updated: 2005-10-05 Contact SGD

References cited on this page View Complete Literature Guide for REG2
1) Frederick DL and Tatchell K  (1996) The REG2 gene of Saccharomyces cerevisiae encodes a type 1 protein phosphatase-binding protein that functions with Reg1p and the Snf1 protein kinase to regulate growth. Mol Cell Biol 16(6):2922-31
2) Jiang H, et al.  (2000) Protein phosphatase type-1 regulatory subunits Reg1p and Reg2p act as signal transducers in the glucose-induced inactivation of maltose permease in Saccharomyces cerevisiae. Mol Gen Genet 263(3):411-22
3) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
4) Lutfiyya LL, et al.  (1998) Characterization of three related glucose repressors and genes they regulate in Saccharomyces cerevisiae. Genetics 150(4):1377-91
5) Haurie V, et al.  (2001) The transcriptional activator Cat8p provides a major contribution to the reprogramming of carbon metabolism during the diauxic shift in Saccharomyces cerevisiae. J Biol Chem 276(1):76-85
6) Gardocki ME, et al.  (2005) Genomic analysis of PIS1 gene expression. Eukaryot Cell 4(3):604-14