SUMMARY PARAGRAPH for REF2
REF2 encodes a nucleic acid-binding protein required for the polyadenylation of pre-mRNAs to normal lengths, and for snoRNA 3'-end maturation (2, 4). Ref2p also participates in mRNA 3'-end maturation prior to the final polyadenylation step (1), and may act as a negative regulator of poly(A) synthesis (4). Ref2p is a component of a large cleavage and polyadenylation factor (CPF)-containing complex (holo-CPF), and is important for recruiting Glc7p and Swd2p to the holo-CPF complex (3). Ref2p is also a component of a holo-CPF subcomplex named APT, for Associated with PTa1p, which primarily associates with the 3'-ends of mRNA-encoding genes and with snoRNA genes, and may be important for preventing readthrough-dependent expression of downstream genes (3).
REF2 is essential for viability in some strain backgrounds (2), and is required for normal growth but not for viability in others, in which ref2 mutants display reduced colony size and vegetative growth rate, abnormal morphologies, and multiple buds (1). ref2 mutants also contain pre-mRNAs with poly(A) tail lengths longer than those produced by wild-type (4).
Last updated: 2005-09-29