RCM1/YNL022C Summary Help

Standard Name RCM1 1, 2
Systematic Name YNL022C
Feature Type ORF, Verified
Description rRNA m5C methyltransferase; methylates cytosine at position 2278 of 25S rRNA while Nop2p methylates cytosine at position 2870; contains seven beta-strand methyltransferase motif; localized to the nucleolus; interacts with Trm112p; homolog of NSUN5A, a human gene which is deleted in Williams-Beuren Syndrome (2, 3, 4, 5, 6)
Chromosomal Location
ChrXIV:592899 to 591427 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All RCM1 GO evidence and references
  View Computational GO annotations for RCM1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 2 genes
Resources
Classical genetics
null
unspecified
Large-scale survey
null
overexpression
Resources
43 total interaction(s) for 43 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 5
  • Affinity Capture-RNA: 1
  • Biochemical Activity: 9

Genetic Interactions
  • Negative Genetic: 22
  • Positive Genetic: 4
  • Synthetic Growth Defect: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 490
Molecular Weight (Da) 56,177
Isoelectric Point (pI) 9.03
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXIV:592899 to 591427 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1997-01-28
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1473 592899..591427 2011-02-03 1997-01-28
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004967
References cited on this page View Complete Literature Guide for RCM1
1) Kos M  (2010) personal communication
2) Sardana R and Johnson AW  (2012) The methyltransferase adaptor protein Trm112 is involved in biogenesis of both ribosomal subunits. Mol Biol Cell 23(21):4313-22
3) Giaever G, et al.  (2002) Functional profiling of the Saccharomyces cerevisiae genome. Nature 418(6896):387-91
4) Kalhor HR, et al.  (2005) A novel methyltransferase required for the formation of the hypermodified nucleoside wybutosine in eucaryotic tRNA. Biochem Biophys Res Commun 334(2):433-40
5) Wade CH, et al.  (2006) The budding yeast rRNA and ribosome biosynthesis (RRB) regulon contains over 200 genes. Yeast 23(4):293-306
6) Sharma S, et al.  (2013) Yeast Nop2 and Rcm1 methylate C2870 and C2278 of the 25S rRNA, respectively. Nucleic Acids Res 41(19):9062-76