RCF1/YML030W Summary Help

Standard Name RCF1 1
Systematic Name YML030W
Alias AIM31 2
Feature Type ORF, Verified
Description Cytochrome c oxidase subunit; required for assembly of the Complex III-Complex IV supercomplex, and for assembly of Cox13p and Rcf2p into cytochrome c oxidase; similar to Rcf2p, and either Rcf1p or Rcf2p is required for late-stage assembly of the Cox12p and Cox13p subunits and for cytochrome c oxidase activity; required for growth under hypoxic conditions; member of the hypoxia induced gene family; C. elegans and human orthologs are functional in yeast (1, 3, 4, 5)
Name Description Respiratory superComplex Factor 1
Chromosomal Location
ChrXIII:216435 to 216914 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All RCF1 GO evidence and references
  View Computational GO annotations for RCF1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 7 genes
Resources
Classical genetics
null
Large-scale survey
null
overexpression
Resources
133 total interaction(s) for 105 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 16
  • Affinity Capture-RNA: 4
  • Affinity Capture-Western: 20
  • Biochemical Activity: 1
  • Co-purification: 1
  • Two-hybrid: 17

Genetic Interactions
  • Dosage Growth Defect: 1
  • Negative Genetic: 60
  • Phenotypic Enhancement: 1
  • Positive Genetic: 7
  • Synthetic Growth Defect: 5

Resources
Expression Summary
histogram
Resources
Length (a.a.) 159
Molecular Weight (Da) 18,477
Isoelectric Point (pI) 9.83
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXIII:216435 to 216914 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 1996-07-31 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..480 216435..216914 1996-07-31 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004492
References cited on this page View Complete Literature Guide for RCF1
1) Strogolova V, et al.  (2012) Rcf1 and Rcf2, members of the hypoxia-induced gene 1 protein family, are critical components of the mitochondrial cytochrome bc1-cytochrome c oxidase supercomplex. Mol Cell Biol 32(8):1363-73
2) Hess DC, et al.  (2009) Computationally driven, quantitative experiments discover genes required for mitochondrial biogenesis. PLoS Genet 5(3):e1000407
3) Helbig AO, et al.  (2009) A three-way proteomics strategy allows differential analysis of yeast mitochondrial membrane protein complexes under anaerobic and aerobic conditions. Proteomics 9(20):4787-98
4) Vukotic M, et al.  (2012) Rcf1 mediates cytochrome oxidase assembly and respirasome formation, revealing heterogeneity of the enzyme complex. Cell Metab 15(3):336-47
5) Chen YC, et al.  (2012) Identification of a protein mediating respiratory supercomplex stability. Cell Metab 15(3):348-60