RAV1/YJR033C Summary Help

Standard Name RAV1 1
Systematic Name YJR033C
Alias SOI3 2 , 3
Feature Type ORF, Verified
Description Subunit of RAVE complex (Rav1p, Rav2p, Skp1p); the RAVE complex promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate (1, 2, 3, 4, 5)
Name Description Regulator of (H+)-ATPase in Vacuolar membrane 1
Chromosomal Location
ChrX:496451 to 492378 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All RAV1 GO evidence and references
Molecular Function
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 5 genes
Resources
Classical genetics
null
unspecified
Large-scale survey
null
Resources
114 total interaction(s) for 70 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 15
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 8
  • Biochemical Activity: 1
  • PCA: 1
  • Reconstituted Complex: 1
  • Two-hybrid: 7

Genetic Interactions
  • Negative Genetic: 44
  • Phenotypic Enhancement: 12
  • Phenotypic Suppression: 10
  • Positive Genetic: 3
  • Synthetic Growth Defect: 7
  • Synthetic Lethality: 3
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 1,357
Molecular Weight (Da) 154,932
Isoelectric Point (pI) 8.2
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrX:496451 to 492378 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..4074 496451..492378 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003794
References cited on this page View Complete Literature Guide for RAV1
1) Seol JH, et al.  (2001) Skp1 forms multiple protein complexes, including RAVE, a regulator of V-ATPase assembly. Nat Cell Biol 3(4):384-91
2) Redding K, et al.  (1996) Allele-specific suppression of a defective trans-Golgi network (TGN) localization signal in Kex2p identifies three genes involved in localization of TGN transmembrane proteins. Mol Cell Biol 16(11):6208-17
3) Sipos G, et al.  (2004) Soi3p/Rav1p functions at the early endosome to regulate endocytic trafficking to the vacuole and localization of trans-Golgi network transmembrane proteins. Mol Biol Cell 15(7):3196-209
4) Ubersax JA, et al.  (2003) Targets of the cyclin-dependent kinase Cdk1. Nature 425(6960):859-64
5) Smardon AM, et al.  (2002) The RAVE complex is essential for stable assembly of the yeast V-ATPase. J Biol Chem 277(16):13831-9