RAM2/YKL019W Summary Help

Standard Name RAM2
Systematic Name YKL019W
Feature Type ORF, Verified
Description Alpha subunit of farnesyltransferase and geranylgeranyltransferase-I; farnesyltransferase (RAM2-RAM1 heterodimer) catalyzes the addition of 15-carbon isoprenoid farnesyl to substrate proteins containing a CAAX consensus motif; type I geranylgeranyltransferase (RAM2-CDC43 heterodimer) catalyzes the addition of the 20-carbon isoprenoid geranylgeranyl to substrate proteins containing a CaaL consensus motif; required for membrane localization of Ras proteins and a-factor (1, 2, 3, 4)
Name Description RAS protein and A-factor Maturation
Chromosomal Location
ChrXI:402568 to 403518 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All RAM2 GO evidence and references
  View Computational GO annotations for RAM2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 65 genes
Resources
Large-scale survey
null
reduction of function
Resources
35 total interaction(s) for 23 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 6
  • Affinity Capture-Western: 1
  • Biochemical Activity: 1
  • Co-purification: 1
  • Two-hybrid: 7

Genetic Interactions
  • Negative Genetic: 10
  • Positive Genetic: 3
  • Synthetic Growth Defect: 4
  • Synthetic Lethality: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 316
Molecular Weight (Da) 37,507
Isoelectric Point (pI) 4.83
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXI:402568 to 403518 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..951 402568..403518 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001502
References cited on this page View Complete Literature Guide for RAM2
1) Mayer ML, et al.  (1992) CDC43 and RAM2 encode the polypeptide subunits of a yeast type I protein geranylgeranyltransferase. J Biol Chem 267(29):20589-93
2) Gomez R, et al.  (1993) Purified yeast protein farnesyltransferase is structurally and functionally similar to its mammalian counterpart. Biochem J 289 ( Pt 1)():25-31
3) He B, et al.  (1991) RAM2, an essential gene of yeast, and RAM1 encode the two polypeptide components of the farnesyltransferase that prenylates a-factor and Ras proteins. Proc Natl Acad Sci U S A 88(24):11373-7
4) Kohl NE, et al.  (1991) Structural homology among mammalian and Saccharomyces cerevisiae isoprenyl-protein transferases. J Biol Chem 266(28):18884-8