RAM1/YDL090C Summary Help

Standard Name RAM1
Systematic Name YDL090C
Alias DPR1 , FUS8 , SCG2 , SGP2 , STE16 1
Feature Type ORF, Verified
Description Beta subunit of the CAAX farnesyltransferase (FTase) that prenylates the a-factor mating pheromone and Ras proteins; required for the membrane localization of Ras proteins and a-factor; homolog of the mammalian FTase beta subunit (2, 3, 4, 5, 6)
Name Description RAS protein and A-factor Maturation 7
Chromosomal Location
ChrIV:296329 to 295034 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Genetic position: -33 cM
Gene Ontology Annotations All RAM1 GO evidence and references
  View Computational GO annotations for RAM1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Mutant phenotypes All RAM1 Phenotype evidence and references
Classical genetics
null
unspecified
Large-scale survey
null
overexpression
Resources
interactions All RAM1 Interaction evidence and references
49 total interaction(s) for 43 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 3
  • Affinity Capture-RNA: 1
  • Two-hybrid: 4
Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Lethality: 1
  • Dosage Rescue: 2
  • Negative Genetic: 27
  • Phenotypic Suppression: 2
  • Positive Genetic: 4
  • Synthetic Growth Defect: 1
  • Synthetic Lethality: 2
  • Synthetic Rescue: 1
Resources
Expression Summary
histogram
Resources
Protein Information All RAM1 Protein evidence and references
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIV:296329 to 295034 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Genetic position: -33 cM
Last Update Coordinates: 2004-02-11 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..1296 296329..295034 2004-02-11 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002248
References cited on this page View Complete Literature Guide for RAM1
1) Wilson KL and Herskowitz I  (1987) STE16, a new gene required for pheromone production by a cells of Saccharomyces cerevisiae. Genetics 115(3):441-9
2) Trueblood CE, et al.  (1997) Substrate specificity determinants in the farnesyltransferase beta-subunit. Proc Natl Acad Sci U S A 94(20):10774-9
3) Gomez R, et al.  (1993) Purified yeast protein farnesyltransferase is structurally and functionally similar to its mammalian counterpart. Biochem J 289 ( Pt 1)():25-31
4) He B, et al.  (1991) RAM2, an essential gene of yeast, and RAM1 encode the two polypeptide components of the farnesyltransferase that prenylates a-factor and Ras proteins. Proc Natl Acad Sci U S A 88(24):11373-7
5) Kohl NE, et al.  (1991) Structural homology among mammalian and Saccharomyces cerevisiae isoprenyl-protein transferases. J Biol Chem 266(28):18884-8
6) Kurth DD, et al.  (1997) Functional consequence of mutating conserved residues of the yeast farnesyl-protein transferase beta-subunit Ram1(Dpr1). Biochemistry 36(50):15932-9
7) Powers S, et al.  (1986) RAM, a gene of yeast required for a functional modification of RAS proteins and for production of mating pheromone a-factor. Cell 47(3):413-22