RAD5/YLR032W Summary Help

RAD5 BASIC INFORMATION

Standard Name RAD5
Systematic Name YLR032W
Alias REV2 1 , SNM2
Feature Type ORF, Verified
Description DNA helicase proposed to promote replication fork regression during postreplication repair by template switching; RING finger containing ubiquitin ligase; stimulates the synthesis of free and PCNA-bound polyubiquitin chains by Ubc13p-Mms2p (2, 3, 4, 5)
Name Description RADiation sensitive
GO Annotations All RAD5 GO evidence and references
    View Computational GO annotations for RAD5
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Mutant Phenotype All RAD5 Phenotype details and references
Classical genetics
null
reduction of function
unspecified
Large-scale survey
null
Interactions RAD5 All interactions details and references
215 total interaction(s) for 110 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 3
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 4
  • Biochemical Activity: 2
  • Reconstituted Complex: 1
  • Two-hybrid: 19
Genetic Interactions
  • Dosage Lethality: 1
  • Dosage Rescue: 1
  • Phenotypic Enhancement: 57
  • Phenotypic Suppression: 8
  • Synthetic Growth Defect: 97
  • Synthetic Lethality: 12
  • Synthetic Rescue: 9
Sequence Information
ChrXII:204992 to 208501 | ORF Map | GBrowse
Gbrowse
Genetic position: 22 cM
Last Update Coordinates: 1996-07-31 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..3510 204992..208501 1996-07-31 1996-07-31
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | UniProtKB
Primary SGDIDS000004022

RAD5 RESOURCES

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  • Functional Analysis

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Expression Summary histogram

ADDITIONAL INFORMATION for RAD5

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for RAD5]

1) Lemontt JF  (1971) Mutants of Yeast Defective in Mutation Induced by Ultraviolet Light. Genetics 68(1):21-33
2) Torres-Ramos CA, et al.  (2002) Requirement of RAD5 and MMS2 for postreplication repair of UV-damaged DNA in Saccharomyces cerevisiae. Mol Cell Biol 22(7):2419-26
3) Kiakos K, et al.  (2002) Saccharomyces cerevisiae RAD5 influences the excision repair of DNA minor groove adducts. J Biol Chem 277(46):44576-81
4) Blastyak A, et al.  (2007) Yeast rad5 protein required for postreplication repair has a DNA helicase activity specific for replication fork regression. Mol Cell 28(1):167-75
5) Carlile CM, et al.  (2009) Synthesis of free and proliferating cell nuclear antigen-bound polyubiquitin chains by the RING E3 ubiquitin ligase Rad5. J Biol Chem 284(43):29326-34