RAD26/YJR035W Summary 
| Standard Name | RAD26 1 |
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| Systematic Name | YJR035W |
| Feature Type | ORF, Verified |
| Description | Protein involved in transcription-coupled nucleotide excision repair of UV-induced DNA lesions; recruitment to DNA lesions is dependent on an elongating RNA polymerase II; homolog of human CSB protein (2, 3 and see Summary Paragraph) |
| Name Description | RADiation sensitive |
| Chromosomal Location | |
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| View Computational GO annotations for RAD26 | |
| Molecular Function | |
| Manually curated | |
| Biological Process | |
| Manually curated | |
| Cellular Component | |
| High-throughput |
| Classical genetics | |
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| overexpression | |
| Large-scale survey | |
| null |
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| overexpression | |
| Resources |
| 134 total interaction(s) for 108 unique genes/features. | |
| Physical Interactions |
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| Genetic Interactions |
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| Resources |
| Localization | |
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| Phosphorylation | PhosphoGRID | PhosphoPep Database |
| Structure | |
| Homologs |
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| Last Update | Coordinates: 2011-02-03 | Sequence: 1996-07-31 | ||||||||||||
| Subfeature details |
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| S288C only | |
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| S288C vs. other species | |
| S288C vs. other strains |
| External Links | All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB |
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| Primary SGDID | S000003796 |
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RAD26 encodes a DNA-dependent ATPase that plays a role in transcription-coupled repair (TCR) (4, 1). Rad26p mediates one of two subpathways of TCR; the second is mediated by Rpb9p, a nonessential subunit of RNA polymerase II (5). The relative contribution of each pathway may depend on the location of the damage relative to a gene (within the coding region vs. near the transcription start site) and its level of transcription (6, 7, 5, 8, 9). In addition to TCR, Rad26p has been shown to be involved in the nucleotide excision repair of non-transcribed regions of the genome and non-transcribed strands of genes (9).
Despite its role in DNA repair, a rad26 mutant does not display sensitivity to DNA-damaging agents such as UV, cisplatin, or X-rays. (1). Depending on the strain background, transcription-coupled repair is not abrograted in rad26 mutants (10). Rad26p has been proposed to affect transcription efficiency during TCR (11, 12, 13, 2). Rad26p forms a complex with the RNA polymerase II degradation factor Def1p when isolated from chromatin, suggesting a connection between DNA damage and ubiquitylation of RNA polymerase II (14, 15).
The RAD26 gene has a human homolog, ERCC6, that is implicated in the severe neurodegerative disorder Cockayne syndrome, type B (1, 4).
Last updated: 2007-07-12 
| 1) | van Gool AJ, et al. (1994) RAD26, the functional S. cerevisiae homolog of the Cockayne syndrome B gene ERCC6. EMBO J 13(22):5361-9 |
| 2) | Lee SK, et al. (2002) Yeast RAD26, a homolog of the human CSB gene, functions independently of nucleotide excision repair and base excision repair in promoting transcription through damaged bases. Mol Cell Biol 22(12):4383-9 |
| 3) | Malik S, et al. (2010) Rad26p, a transcription-coupled repair factor, is recruited to the site of DNA lesion in an elongating RNA polymerase II-dependent manner in vivo. Nucleic Acids Res 38(5):1461-77 |
| 4) | Guzder SN, et al. (1996) RAD26, the yeast homolog of human Cockayne's syndrome group B gene, encodes a DNA-dependent ATPase. J Biol Chem 271(31):18314-7 |
| 5) | Li S and Smerdon MJ (2002) Rpb4 and Rpb9 mediate subpathways of transcription-coupled DNA repair in Saccharomyces cerevisiae. EMBO J 21(21):5921-9 |
| 6) | Teng Y and Waters R (2000) Excision repair at the level of the nucleotide in the upstream control region, the coding sequence and in the region where transcription terminates of the Saccharomyces cerevisiae MFA2 gene and the role of RAD26. Nucleic Acids Res 28(5):1114-9 |
| 7) | van den Boom V, et al. (2002) When machines get stuck--obstructed RNA polymerase II: displacement, degradation or suicide. Bioessays 24(9):780-4 |
| 8) | Li S and Smerdon MJ (2004) Dissecting transcription-coupled and global genomic repair in the chromatin of yeast GAL1-10 genes. J Biol Chem 279(14):14418-26 |
| 9) | Li S, et al. (2006) Modulation of Rad26- and Rpb9-mediated DNA repair by different promoter elements. J Biol Chem 281(48):36643-51 |
| 10) | Gregory SM and Sweder KS (2001) Deletion of the CSB homolog, RAD26, yields Spt(-) strains with proficient transcription-coupled repair. Nucleic Acids Res 29(14):3080-6 |
| 11) | You Z, et al. (1998) Yeast RNA polymerase II transcription in vitro is inhibited in the presence of nucleotide excision repair: complementation of inhibition by Holo-TFIIH and requirement for RAD26. Mol Cell Biol 18(5):2668-76 |
| 12) | Tijsterman M and Brouwer J (1999) Rad26, the yeast homolog of the cockayne syndrome B gene product, counteracts inhibition of DNA repair due to RNA polymerase II transcription. J Biol Chem 274(3):1199-202 |
| 13) | Lee SK, et al. (2001) Requirement for yeast RAD26, a homolog of the human CSB gene, in elongation by RNA polymerase II. Mol Cell Biol 21(24):8651-6 |
| 14) | Woudstra EC, et al. (2002) A Rad26-Def1 complex coordinates repair and RNA pol II proteolysis in response to DNA damage. Nature 415(6874):929-33 |
| 15) | Reid J and Svejstrup JQ (2004) DNA damage-induced Def1-RNA polymerase II interaction and Def1 requirement for polymerase ubiquitylation in vitro. J Biol Chem 279(29):29875-8 |
