RAD16/YBR114W Summary Help

Standard Name RAD16 1
Systematic Name YBR114W
Alias PSO5
Feature Type ORF, Verified
Description Nucleotide excision repair (NER) protein; binds damaged DNA during NER; binds DNA in an ATP-dependent manner (with Rad7p) during NER; required for NER of non-transcribed chromatin; subunit of Nucleotide Excision Repair Factor 4 (NEF4) and the Elongin-Cullin-Socs (ECS) ligase complex (2, 3, 4 and see Summary Paragraph)
Name Description RADiation sensitive
Chromosomal Location
ChrII:467248 to 469620 | ORF Map | GBrowse
Gbrowse
Genetic position: 58 cM
Gene Ontology Annotations All RAD16 GO evidence and references
  View Computational GO annotations for RAD16
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 5 genes
Resources
Classical genetics
null
reduction of function
unspecified
Large-scale survey
null
Resources
110 total interaction(s) for 70 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 37
  • Affinity Capture-Western: 8
  • Co-fractionation: 2
  • Co-purification: 6
  • Reconstituted Complex: 2
  • Two-hybrid: 7

Genetic Interactions
  • Dosage Lethality: 1
  • Negative Genetic: 12
  • Phenotypic Enhancement: 17
  • Phenotypic Suppression: 2
  • Positive Genetic: 5
  • Synthetic Growth Defect: 6
  • Synthetic Lethality: 3
  • Synthetic Rescue: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 790
Molecular Weight (Da) 91,430
Isoelectric Point (pI) 8.27
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrII:467248 to 469620 | ORF Map | GBrowse
SGD ORF map
Genetic position: 58 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1997-01-28
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2373 467248..469620 2011-02-03 1997-01-28
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000318
SUMMARY PARAGRAPH for RAD16

RAD16 encodes a protein that acts in nucleotide excision repair (NER) of UV-damaged DNA. In NER, damaged DNA is excised by incision of the DNA on either side of the lesion followed by unwinding by DNA helicases; the process of NER is reviewed in 5. Rad16p and Rad7p form a stoichiometric complex called NEF4 (6). NEF4 binds specifically to UV-damaged DNA in an ATP-dependent manner (6), and has DNA-dependent ATPase activity (7). Rad4p and Rad23p form another complex, called NEF2, that binds cooperatively with NEF4 to damaged DNA (8). Unlike NEF2, NEF4 is not required for incision, but the in vitro incision reaction is enhanced when both NEF2 and NEF4 are present (6, 8). rad16 mutants, like rad7 mutants, are proficient in incision but defective in both oligonucleotide excision and repair synthesis, suggesting that Rad7p and Rad16p act in a post-incision step of NER (9).

In humans, NER deficiencies are associated with xeroderma pigmentosum (XP) and Cockayne syndrome (5, 10). Homologs of Rad7p and Rad16p have been identified in S. pombe (11).

Last updated: 1999-11-18 Contact SGD

References cited on this page View Complete Literature Guide for RAD16
1) Game, J.  (1989) Personal Communication, Mortimer Map Edition 10
2) Prakash S and Prakash L  (2000) Nucleotide excision repair in yeast. Mutat Res 451(1-2):13-24
3) Gillette TG, et al.  (2006) Distinct functions of the ubiquitin-proteasome pathway influence nucleotide excision repair. EMBO J 25(11):2529-38
4) Lettieri T, et al.  (2008) Functionally distinct nucleosome-free regions in yeast require Rad7 and Rad16 for nucleotide excision repair. DNA Repair (Amst) 7(5):734-43
5) Friedberg EC, et al.  (1995) DNA Repair and Mutagenesis. Washington, DC: American Society for Microbiology (ASM) Press
6) Guzder SN, et al.  (1997) Yeast Rad7-Rad16 complex, specific for the nucleotide excision repair of the nontranscribed DNA strand, is an ATP-dependent DNA damage sensor. J Biol Chem 272(35):21665-8
7) Guzder SN, et al.  (1998) The DNA-dependent ATPase activity of yeast nucleotide excision repair factor 4 and its role in DNA damage recognition. J Biol Chem 273(11):6292-6
8) Guzder SN, et al.  (1999) Synergistic interaction between yeast nucleotide excision repair factors NEF2 and NEF4 in the binding of ultraviolet-damaged DNA. J Biol Chem 274(34):24257-62
9) Reed SH, et al.  (1998) The yeast RAD7 and RAD16 genes are required for postincision events during nucleotide excision repair. In vitro and in vivo studies with rad7 and rad16 mutants and purification of a Rad7/Rad16-containing protein complex. J Biol Chem 273(45):29481-8
10) Foury F  (1997) Human genetic diseases: a cross-talk between man and yeast. Gene 195(1):1-10
11) Lombaerts M, et al.  (1999) Characterization of the rhp7(+) and rhp16(+) genes in Schizosaccharomyces pombe. Nucleic Acids Res 27(17):3410-6