PYK2/YOR347C Summary Help

Standard Name PYK2
Systematic Name YOR347C
Feature Type ORF, Verified
Description Pyruvate kinase; appears to be modulated by phosphorylation; transcription repressed by glucose, and Pyk2p may be active under low glycolytic flux; PYK2 has a paralog, CDC19, that arose from the whole genome duplication (1, 2, 3 and see Summary Paragraph)
Name Description PYruvate Kinase
Chromosomal Location
ChrXV:986462 to 984942 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All PYK2 GO evidence and references
  View Computational GO annotations for PYK2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 4 genes
Large-scale survey
29 total interaction(s) for 25 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 3
  • Affinity Capture-RNA: 3
  • Biochemical Activity: 3
  • Reconstituted Complex: 2
  • Two-hybrid: 2

Genetic Interactions
  • Negative Genetic: 7
  • Positive Genetic: 5
  • Synthetic Growth Defect: 1
  • Synthetic Lethality: 1
  • Synthetic Rescue: 2

Expression Summary
Length (a.a.) 506
Molecular Weight (Da) 55,195
Isoelectric Point (pI) 6.9
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXV:986462 to 984942 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1521 986462..984942 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005874

PYK2 is one of two S. cerevisiae genes encoding pyruvate kinase which catalyzes the conversion of phosphoenolpyruvate to pyruvate (1). Deletion of PYK2 causes no obvious growth phenotype, but a pyk2 mae1 double deletion mutant cannot grow using ethanol as the sole carbon source; MAE1 encodes malic enzyme which catalyzes the oxidative decarboxylation of malate to pyruvate (4).

Deletion of both PYK2 and CDC19, the other pyruvate kinase gene in yeast, causes a more severe growth defect than a cdc19 single deletion (1). Overexpression of PYK2 restores growth on glucose to cdc19 mutant cells (1). However, CDC19 is tightly regulated and activated by fructose-1,6-bisphosphate (FBP) whereas PYK2 is subject to glucose repression and appears to be insensitive to FBP levels, suggesting that it may be active when FBP levels are too low to activate CDC19 (1). Therefore, PYK1 appears to be the main pyruvate kinase in the glycolytic pathway (1, 5, 6).

Last updated: 2005-07-28 Contact SGD

References cited on this page View Complete Literature Guide for PYK2
1) Boles E, et al.  (1997) Characterization of a glucose-repressed pyruvate kinase (Pyk2p) in Saccharomyces cerevisiae that is catalytically insensitive to fructose-1,6-bisphosphate. J Bacteriol 179(9):2987-93
2) Portela P, et al.  (2002) In vivo and in vitro phosphorylation of two isoforms of yeast pyruvate kinase by protein kinase A. J Biol Chem 277(34):30477-87
3) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
4) Boles E, et al.  (1998) Identification and characterization of MAE1, the Saccharomyces cerevisiae structural gene encoding mitochondrial malic enzyme. J Bacteriol 180(11):2875-82
5) Sprague GF Jr  (1977) Isolation and characterization of a Saccharomyces cerevisiae mutant deficient in pyruvate kinase activity. J Bacteriol 130(1):232-41
6) Fraenkel DG  (2003) The top genes: on the distance from transcript to function in yeast glycolysis. Curr Opin Microbiol 6(2):198-201