PYK2/YOR347C Summary

Standard Name	PYK2
Systematic Name	YOR347C
Feature Type	ORF, Verified
Description	Pyruvate kinase; appears to be modulated by phosphorylation; transcription repressed by glucose, and Pyk2p may be active under low glycolytic flux; PYK2 has a paralog, CDC19, that arose from the whole genome duplication (1, 2, 3 and see Summary Paragraph)
Name Description	PYruvate Kinase

Chromosomal Location	ChrXV:986462 to 984942 \| ORF Map \| GBrowse
	Note: this feature is encoded on the Crick strand.

Gene Ontology Annotations All PYK2 GO evidence and references

	View Computational GO annotations for PYK2
Molecular Function
Manually curated	pyruvate kinase activity (IMP)
Biological Process
Manually curated	pyruvate metabolic process (IMP)
Cellular Component
Manually curated	mitochondrion (IDA)
High-throughput	cytoplasm (IDA)

Pathways	glucose fermentation glycolysis

Mutant phenotypes All PYK2 Phenotype evidence and references

Large-scale survey
null	polyphosphate accumulation: abnormal competitive fitness: increased vacuolar morphology: abnormal viable
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All PYK2 Interaction evidence and references

	28 total interaction(s) for 24 unique genes/features.
Physical Interactions	Affinity Capture-MS: 3 Affinity Capture-RNA: 2 Biochemical Activity: 3 Reconstituted Complex: 2 Two-hybrid: 2
Genetic Interactions	Negative Genetic: 7 Positive Genetic: 5 Synthetic Growth Defect: 1 Synthetic Lethality: 1 Synthetic Rescue: 2
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All PYK2 Protein evidence and references

Localization	YeastRC \| Organelle DB (UMich) \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrXV:986462 to 984942 | |

Note: this feature is encoded on the Crick strand.

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..1521	986462..984942	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| E.C. \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000005874

SUMMARY PARAGRAPH for PYK2

PYK2 is one of two S. cerevisiae genes encoding pyruvate kinase which catalyzes the conversion of phosphoenolpyruvate to pyruvate (1). Deletion of PYK2 causes no obvious growth phenotype, but a pyk2 mae1 double deletion mutant cannot grow using ethanol as the sole carbon source; MAE1 encodes malic enzyme which catalyzes the oxidative decarboxylation of malate to pyruvate (4).

Deletion of both PYK2 and CDC19, the other pyruvate kinase gene in yeast, causes a more severe growth defect than a cdc19 single deletion (1). Overexpression of PYK2 restores growth on glucose to cdc19 mutant cells (1). However, CDC19 is tightly regulated and activated by fructose-1,6-bisphosphate (FBP) whereas PYK2 is subject to glucose repression and appears to be insensitive to FBP levels, suggesting that it may be active when FBP levels are too low to activate CDC19 (1). Therefore, PYK1 appears to be the main pyruvate kinase in the glycolytic pathway (1, 5, 6).

Last updated: 2005-07-28

References cited on this page View Complete Literature Guide for PYK2

1)	Boles E, et al. (1997) Characterization of a glucose-repressed pyruvate kinase (Pyk2p) in Saccharomyces cerevisiae that is catalytically insensitive to fructose-1,6-bisphosphate. J Bacteriol 179(9):2987-93
2)	Portela P, et al. (2002) In vivo and in vitro phosphorylation of two isoforms of yeast pyruvate kinase by protein kinase A. J Biol Chem 277(34):30477-87
3)	Byrne KP and Wolfe KH (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
4)	Boles E, et al. (1998) Identification and characterization of MAE1, the Saccharomyces cerevisiae structural gene encoding mitochondrial malic enzyme. J Bacteriol 180(11):2875-82
5)	Sprague GF Jr (1977) Isolation and characterization of a Saccharomyces cerevisiae mutant deficient in pyruvate kinase activity. J Bacteriol 130(1):232-41
6)	Fraenkel DG (2003) The top genes: on the distance from transcript to function in yeast glycolysis. Curr Opin Microbiol 6(2):198-201