Standard Name	PUS4
Systematic Name	YNL292W
Feature Type	ORF, Verified
Description	Pseudouridine synthase, catalyzes only the formation of pseudouridine-55 (Psi55), a highly conserved tRNA modification, in mitochondrial and cytoplasmic tRNAs; PUS4 overexpression leads to translational derepression of GCN4 (Gcd- phenotype) (1, 2)
Name Description	PseudoUridine Synthase

Chromosomal Location	ChrXIV:82806 to 84017 | ORF Map | GBrowse

	View Computational GO annotations for PUS4
Molecular Function
Manually curated	pseudouridine synthase activity (IDA, IGI)
Biological Process
Manually curated	tRNA modification (IDA)
Cellular Component
Manually curated	mitochondrion (IMP) nucleus (IMP)

Classical genetics
null	resistance to tellurite: increased
Large-scale survey
null	competitive fitness: decreased resistance to cycloheximide: decreased resistance to paromomycin: decreased resistance to tirapazamine: increased resistance to benzo[a]pyrene: decreased viable
overexpression	vegetative growth: decreased rate vegetative growth: decreased
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	34 total interaction(s) for 31 unique genes/features.
Physical Interactions	Affinity Capture-MS: 3 Affinity Capture-RNA: 2 Reconstituted Complex: 2 Two-hybrid: 1
Genetic Interactions	Dosage Lethality: 1 Dosage Rescue: 1 Negative Genetic: 23 Synthetic Growth Defect: 1
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | AspGD Homologs | CGD Homologs | CandidaDB Homologs | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrXIV:82806 to 84017 | ORF Map | GBrowse
Last Update	Coordinates: 2005-11-07 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..1212 82806..84017 2005-11-07 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI1631 S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain CLIB215 S.c. Strain CLIB324 S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain M22 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain T73 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000005236