Standard Name	PUS1
Systematic Name	YPL212C
Feature Type	ORF, Verified
Description	tRNA:pseudouridine synthase; introduces pseudouridines at positions 26-28, 34-36, 65, and 67 of tRNA; nuclear protein that appears to be involved in tRNA export; also acts on U2 snRNA; PUS1 has a paralog, PUS2, that arose from the whole genome duplication (1, 2, 3)
Name Description	PseudoUridine Synthase

Chromosomal Location	ChrXVI:153149 to 151515 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.

	View Computational GO annotations for PUS1
Molecular Function
Manually curated	pseudouridine synthase activity (IDA)
Biological Process
Manually curated	snRNA pseudouridine synthesis (IMP) tRNA pseudouridine synthesis (IDA)
Cellular Component
Manually curated	nucleus (IPI)

Classical genetics
null	heat sensitivity: increased resistance to 5-fluorouracil: decreased
Large-scale survey
null	competitive fitness: decreased endocytosis: decreased heat sensitivity: increased hyperosmotic stress resistance: decreased resistance to sirolimus: decreased resistance to 5-fluorouracil: decreased resistance to doxorubicin: decreased resistance to ethanol: decreased viable
overexpression	vegetative growth: decreased rate
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	54 total interaction(s) for 41 unique genes/features.
Physical Interactions	Affinity Capture-MS: 8 Affinity Capture-RNA: 3 PCA: 2
Genetic Interactions	Dosage Lethality: 1 Negative Genetic: 20 Phenotypic Enhancement: 1 Phenotypic Suppression: 1 Positive Genetic: 5 Synthetic Growth Defect: 11 Synthetic Lethality: 2
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | AspGD Homologs | CGD Homologs | CandidaDB Homologs | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrXVI:153149 to 151515 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.
Last Update	Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..1635 153149..151515 2011-02-03 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI1631 S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain CLIB215 S.c. Strain EC1118 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain YPS163 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000006133