PUS1/YPL212C Summary Help

Standard Name PUS1
Systematic Name YPL212C
Feature Type ORF, Verified
Description tRNA:pseudouridine synthase; introduces pseudouridines at positions 26-28, 34-36, 65, and 67 of tRNA; also acts on U2 snRNA; also pseudouridylates some mRNAs, and pseudouridylation level varies with growth phase; nuclear protein that appears to be involved in tRNA export; PUS1 has a paralog, PUS2, that arose from the whole genome duplication (1, 2, 3, 4)
Name Description PseudoUridine Synthase
Chromosomal Location
ChrXVI:153149 to 151515 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All PUS1 GO evidence and references
  View Computational GO annotations for PUS1
Molecular Function
Manually curated
High-throughput
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 3 genes
Resources
Classical genetics
null
Large-scale survey
null
overexpression
Resources
57 total interaction(s) for 41 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 10
  • Affinity Capture-RNA: 4
  • PCA: 2

Genetic Interactions
  • Dosage Lethality: 1
  • Negative Genetic: 20
  • Phenotypic Enhancement: 1
  • Phenotypic Suppression: 1
  • Positive Genetic: 5
  • Synthetic Growth Defect: 11
  • Synthetic Lethality: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 544
Molecular Weight (Da) 62,142
Isoelectric Point (pI) 8.31
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXVI:153149 to 151515 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1635 153149..151515 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000006133
References cited on this page View Complete Literature Guide for PUS1
1) Massenet S, et al.  (1999) Pseudouridine mapping in the Saccharomyces cerevisiae spliceosomal U small nuclear RNAs (snRNAs) reveals that pseudouridine synthase pus1p exhibits a dual substrate specificity for U2 snRNA and tRNA. Mol Cell Biol 19(3):2142-54
2) Grosshans H, et al.  (2001) Pus1p-dependent tRNA pseudouridinylation becomes essential when tRNA biogenesis is compromised in yeast. J Biol Chem 276(49):46333-9
3) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
4) Carlile TM, et al.  (2014) Pseudouridine profiling reveals regulated mRNA pseudouridylation in yeast and human cells.LID - 10.1038/nature13802 [doi] Nature ()