PUN1/YLR414C Summary Help

Standard Name PUN1 1
Systematic Name YLR414C
Feature Type ORF, Verified
Description Plasma membrane protein with a role in cell wall integrity; co-localizes with Sur7p in punctate membrane patches; null mutant displays decreased thermotolerance; transcription induced upon cell wall damage and metal ion stress (2, 3, 4, 5)
Name Description Plasma membrane protein Upregulated during Nitrogen stress 1
Chromosomal Location
ChrXII:954144 to 953353 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All PUN1 GO evidence and references
  View Computational GO annotations for PUN1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 11 genes
Resources
Classical genetics
null
Large-scale survey
null
overexpression
Resources
24 total interaction(s) for 19 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 4
  • Affinity Capture-RNA: 5
  • Affinity Capture-Western: 1
  • Protein-RNA: 1
  • Reconstituted Complex: 1

Genetic Interactions
  • Negative Genetic: 8
  • Positive Genetic: 4

Resources
Expression Summary
histogram
Resources
Length (a.a.) 263
Molecular Weight (Da) 29,251
Isoelectric Point (pI) 8.54
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXII:954144 to 953353 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..792 954144..953353 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004406
References cited on this page View Complete Literature Guide for PUN1
1) Xu T, et al.  (2010) A profile of differentially abundant proteins at the yeast cell periphery during pseudohyphal growth. J Biol Chem 285(20):15476-88
2) Huh WK, et al.  (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91
3) Rodriguez-Pena JM, et al.  (2005) The 'yeast cell wall chip' - a tool to analyse the regulation of cell wall biogenesis in Saccharomyces cerevisiae. Microbiology 151(Pt 7):2241-9
4) Alvarez FJ, et al.  (2008) The Sur7 protein regulates plasma membrane organization and prevents intracellular cell wall growth in Candida albicans. Mol Biol Cell 19(12):5214-25
5) Mir SS, et al.  (2009) Ssd1 is required for thermotolerance and Hsp104-mediated protein disaggregation in Saccharomyces cerevisiae. Mol Cell Biol 29(1):187-200