PUF6/YDR496C Summary Help

Standard Name PUF6
Systematic Name YDR496C
Feature Type ORF, Verified
Description Pumilio-homology domain protein; binds the 3' UTR of ASH1 mRNA and represses its translation, resulting in proper asymmetric localization of ASH1 mRNA; required at post-transcriptional step for efficient retrotransposition; absence results in decreased Ty1 Gag:GFP protein levels; co-sediments with the 60S ribosomal subunit and is required for its biogenesis (1, 2, 3)
Name Description PUmilio-homology domain Family
Chromosomal Location
ChrIV:1443403 to 1441433 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All PUF6 GO evidence and references
  View Computational GO annotations for PUF6
Molecular Function
Manually curated
High-throughput
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 2 genes
Resources
Classical genetics
null
Large-scale survey
null
overexpression
unspecified
Resources
227 total interaction(s) for 154 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 147
  • Affinity Capture-RNA: 4
  • Affinity Capture-Western: 4
  • Biochemical Activity: 1
  • Co-fractionation: 1
  • Reconstituted Complex: 1

Genetic Interactions
  • Dosage Lethality: 1
  • Negative Genetic: 39
  • Phenotypic Enhancement: 2
  • Positive Genetic: 20
  • Synthetic Growth Defect: 4
  • Synthetic Lethality: 2
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 656
Molecular Weight (Da) 75,105
Isoelectric Point (pI) 6.88
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIV:1443403 to 1441433 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1971 1443403..1441433 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002904
References cited on this page View Complete Literature Guide for PUF6
1) Gu W, et al.  (2004) A new yeast PUF family protein, Puf6p, represses ASH1 mRNA translation and is required for its localization. Genes Dev 18(12):1452-65
2) Li Z, et al.  (2009) Rational extension of the ribosome biogenesis pathway using network-guided genetics. PLoS Biol 7(10):e1000213
3) Risler JK, et al.  (2012) Host co-factors of the retrovirus-like transposon Ty1. Mob DNA 3(1):12