PUF4/YGL014W Summary

PUF4 BASIC INFORMATION

Standard Name	PUF4 1
Systematic Name	YGL014W
Alias	YGL023 2
Feature Type	ORF, Verified
Description	Member of the PUF protein family, which is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding nucleolar ribosomal RNA-processing factors (3)
Name Description	PUmilio-homology domain Family

GO Annotations	All PUF4 GO evidence and references
	View Computational GO annotations for PUF4
Molecular Function
Manually curated	mRNA binding (IDA, ISS)
High-throughput	mRNA binding (IDA)
Biological Process
Manually curated	loss of chromatin silencing during replicative cell aging (IGI, IMP) nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay (IGI) protein localization (IMP)
Cellular Component
High-throughput	cytoplasm (IDA)

Mutant Phenotype	All PUF4 Phenotype details and references
Classical genetics
overexpression	budding: abnormal cell cycle progression in G1 phase: abnormal
Large-scale survey
null	filamentous growth: decreased invasive growth: absent viable
overexpression	inviable

Interactions	PUF4 All interactions details and references
	93 total interaction(s) for 93 unique genes/features.
Physical Interactions	Affinity Capture-MS: 44 Affinity Capture-RNA: 2 Affinity Capture-Western: 2 Biochemical Activity: 4 Co-crystal Structure: 1 PCA: 8 Two-hybrid: 1
Genetic Interactions	Phenotypic Enhancement: 24 Phenotypic Suppression: 6 Synthetic Lethality: 1

Sequence Information

ChrVII:466146 to 468812 | |

This feature contains embedded feature(s): YGL014C-A

Last Update

Coordinates: 2004-07-20 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..2667	466146..468812	2004-07-20	1996-07-31

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| UniProtKB

Primary SGDID	S000002982

PUF4 RESOURCES

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SGD ORF map	GBrowse

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Expression Summary

ADDITIONAL INFORMATION for PUF4

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for PUF4]

1)	Olivas W and Parker R (2000) The Puf3 protein is a transcript-specific regulator of mRNA degradation in yeast. EMBO J 19(23):6602-11
2)	Chen WN, et al. (1991) The YGL023 gene encodes a putative regulatory protein. Yeast 7(3):309-12
3)	Gerber AP, et al. (2004) Extensive association of functionally and cytotopically related mRNAs with Puf family RNA-binding proteins in yeast. PLoS Biol 2(3):E79

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