PUB1/YNL016W Summary Help

Standard Name PUB1 1
Systematic Name YNL016W
Alias RNP1 2
Feature Type ORF, Verified
Description Poly (A)+ RNA-binding protein; abundant mRNP-component protein that binds mRNA and is required for stability of many mRNAs; component of glucose deprivation induced stress granules, involved in P-body-dependent granule assembly; protein abundance increases in response to DNA replication stress (1, 3, 4, 5, 6)
Name Description PolyUridylate Binding 1
Chromosomal Location
ChrXIV:602907 to 604268 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All PUB1 GO evidence and references
  View Computational GO annotations for PUB1
Molecular Function
Manually curated
High-throughput
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 3 genes
Resources
Classical genetics
null
overexpression
Large-scale survey
null
Resources
147 total interaction(s) for 120 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 22
  • Affinity Capture-RNA: 4
  • Affinity Capture-Western: 3
  • Co-fractionation: 1
  • PCA: 15
  • Reconstituted Complex: 3
  • Two-hybrid: 5

Genetic Interactions
  • Negative Genetic: 74
  • Phenotypic Suppression: 1
  • Positive Genetic: 11
  • Synthetic Growth Defect: 7
  • Synthetic Lethality: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 453
Molecular Weight (Da) 50,763
Isoelectric Point (pI) 4.81
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXIV:602907 to 604268 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1997-01-28
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1362 602907..604268 2011-02-03 1997-01-28
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004961
References cited on this page View Complete Literature Guide for PUB1
1) Matunis MJ, et al.  (1993) PUB1: a major yeast poly(A)+ RNA-binding protein. Mol Cell Biol 13(10):6114-23
2) Ripmaster TL and Woolford JL Jr  (1993) A protein containing conserved RNA-recognition motifs is associated with ribosomal subunits in Saccharomyces cerevisiae. Nucleic Acids Res 21(14):3211-6
3) Anderson JT, et al.  (1993) PUB1 is a major nuclear and cytoplasmic polyadenylated RNA-binding protein in Saccharomyces cerevisiae. Mol Cell Biol 13(10):6102-13
4) Duttagupta R, et al.  (2005) Global analysis of Pub1p targets reveals a coordinate control of gene expression through modulation of binding and stability. Mol Cell Biol 25(13):5499-513
5) Buchan JR, et al.  (2008) P bodies promote stress granule assembly in Saccharomyces cerevisiae. J Cell Biol 183(3):441-55
6) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76