PTA1/YAL043C Summary Help

Standard Name PTA1 1
Systematic Name YAL043C
Alias FUN39
Feature Type ORF, Verified
Description Subunit of holo-CPF; holo-CPF is a multiprotein complex and functional homolog of mammalian CPSF, required for the cleavage and polyadenylation of mRNA and snoRNA 3' ends; involved in pre-tRNA processing; binds to the phosphorylated CTD of RNAPII (1, 2, 3, 4, 5)
Name Description Pre-Trna Accumulation 1
Chromosomal Location
ChrI:61052 to 58695 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All PTA1 GO evidence and references
  View Computational GO annotations for PTA1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 8 genes
Resources
Classical genetics
conditional
null
Large-scale survey
conditional
null
reduction of function
repressible
Resources
270 total interaction(s) for 113 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 159
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 9
  • Biochemical Activity: 3
  • Co-purification: 1
  • Far Western: 1
  • Reconstituted Complex: 16
  • Two-hybrid: 5

Genetic Interactions
  • Dosage Lethality: 2
  • Dosage Rescue: 2
  • Negative Genetic: 67
  • Synthetic Lethality: 2
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 785
Molecular Weight (Da) 88,472
Isoelectric Point (pI) 6.17
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrI:61052 to 58695 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2358 61052..58695 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000041
References cited on this page View Complete Literature Guide for PTA1
1) O'Connor JP and Peebles CL  (1992) PTA1, an essential gene of Saccharomyces cerevisiae affecting pre-tRNA processing. Mol Cell Biol 12(9):3843-56
2) Preker PJ, et al.  (1997) A multisubunit 3' end processing factor from yeast containing poly(A) polymerase and homologues of the subunits of mammalian cleavage and polyadenylation specificity factor. EMBO J 16(15):4727-37
3) Zhao J, et al.  (1999) Pta1, a component of yeast CF II, is required for both cleavage and poly(A) addition of mRNA precursor. Mol Cell Biol 19(11):7733-40
4) Rodriguez CR, et al.  (2000) Kin28, the TFIIH-associated carboxy-terminal domain kinase, facilitates the recruitment of mRNA processing machinery to RNA polymerase II. Mol Cell Biol 20(1):104-12
5) Nedea E, et al.  (2003) Organization and function of APT, a subcomplex of the yeast cleavage and polyadenylation factor involved in the formation of mRNA and small nucleolar RNA 3'-ends. J Biol Chem 278(35):33000-10