PSY4/YBL046W Summary Help

Standard Name PSY4 1
Systematic Name YBL046W
Feature Type ORF, Verified
Description Regulatory subunit of protein phosphatase PP4; presence of Psy4p in the PP4 complex (along with catalytic subunit Pph3p and Psy2p) is required for dephosphorylation of the histone variant H2AX, but not for dephosphorylation of Rad53p, during recovery from the DNA damage checkpoint; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation; required for cisplatin resistance; homolog of mammalian R2 (1, 2, 3, 4)
Name Description Platinum SensitivitY 5
Chromosomal Location
ChrII:132424 to 133749 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All PSY4 GO evidence and references
  View Computational GO annotations for PSY4
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 6 genes
Resources
Classical genetics
null
Large-scale survey
null
Resources
76 total interaction(s) for 35 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 42
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 5
  • Two-hybrid: 5

Genetic Interactions
  • Negative Genetic: 9
  • Phenotypic Suppression: 7
  • Positive Genetic: 5
  • Synthetic Growth Defect: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 441
Molecular Weight (Da) 50,655
Isoelectric Point (pI) 4.09
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrII:132424 to 133749 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1997-01-28
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1326 132424..133749 2011-02-03 1997-01-28
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000142
References cited on this page View Complete Literature Guide for PSY4
1) Hastie CJ, et al.  (2006) The Saccharomyces cerevisiae orthologue of the human protein phosphatase 4 core regulatory subunit R2 confers resistance to the anticancer drug cisplatin. FEBS J 273(14):3322-34
2) Keogh MC, et al.  (2006) A phosphatase complex that dephosphorylates gammaH2AX regulates DNA damage checkpoint recovery. Nature 439(7075):497-501
3) O'Neill BM, et al.  (2007) Pph3-Psy2 is a phosphatase complex required for Rad53 dephosphorylation and replication fork restart during recovery from DNA damage. Proc Natl Acad Sci U S A 104(22):9290-5
4) Kosugi S, et al.  (2009) Systematic identification of cell cycle-dependent yeast nucleocytoplasmic shuttling proteins by prediction of composite motifs. Proc Natl Acad Sci U S A 106(25):10171-6
5) Wu HI, et al.  (2004) Genome-wide identification of genes conferring resistance to the anticancer agents cisplatin, oxaliplatin, and mitomycin C. Cancer Res 64(11):3940-8